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On-Line Enrichment and Determination of Antihypertensives in Human Biological Fluid Using Weak Cation-Exchange Monolithic Column

Author: ZhangZuo
Tutor: YangGengLiang
School: Hebei University
Course: Pharmaceutical Analysis
Keywords: weak cation-exchange monolithic column deproteinization on-line enrichment biological samples
CLC: O657.75
Type: Master's thesis
Year: 2007
Downloads: 17
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Abstract


Ion-exchange monolithic columns were developed greatly in recent years. The advantages of good hydrodynamic characteristic, low flow resistance as well as easy preparation made monolithic material an ideal support for the purification of biomacromolecules. Monolithic material had been widely used in separation and purification of protein, peptide, nucleic acid, polyribonucleotide, and polysaccharide. Furthermore, they could also be used as solid phase extraction (SPE) material in the preconcentration of trace components in biological sample before further analysis. Compared to conventional enrichment techniques, it is safe, convenient, effective and making automatic on-line analysis possible without overfull consumption of organic solvent.In the paper, the weak cation-exchange monolithic column was prepared by "in-situ" polymerization, and ion-exchange groups were bonded on the column by "on-line" modification. It was investigated that simultaneous deproteinization and enrichment of drugs in biological samples using the monolithic column as SPE material. There were four chapters in this thesis.Chapter one:A review was mainly concerned with the principle and the recent progress in the stationary phase of ion-exchange chromatography (IEC); also, the basis, the setting, the procedure as well as sorts of SPE were introduced.Chapter two:A weak cation-exchange monolithic column was prepared by modifying the GMA-EDMA monoliths with ethylenediamine and monochloracetic acid. The properties of the column were investigated, and low backpressure and fast analysis could be obtained on the column. The on-line sample deproteinization was carried out, and the enrichment of drugs had been completed at the same time on the monolithic column. Flunarizine was determined in human plasma using the weak cation-exchange monolithic column as pretreatment material. The method to assay drugs in the blood was simple, effective and friendly to environment. No obvious changes happened on the column efficiency and back pressure within two months.Chapter three:The weak ion exchange monolithic material for on-line clean-up and determination of cinnarizine in human urine was investigated.Chapter four:Prazosin and doxazosin were simultaneously determined in human urine using the weak cation-exchange monolith column as solid phase extraction material.

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CLC: > Mathematical sciences and chemical > Chemistry > Analytical Chemistry > Instrument analysis ( physics and physical chemistry ) > Chromatographic analysis > Ion exchange chromatography
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