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In this study, four goats installed with permanent rumen flstulae were used in three experiments to evaluate effect of hainanmycin on ruminal digestive metabolism and biohydrogenation both in vitro and in vivo.1 Effect of hainanmycin on ruminal digestive emtabolism in vitroFour adult goats installed with permanent rumen fistulae were used as donators of rumen liquid, and effect of hainanmycin on ruminal digestive emtabolism was investigated in vitro. Samples were assigned in 4 groups, including 1 control,3 groups treated with hainanmycin (2.5mg/L,5mg/L and 10mg/L). The pH value and concentrations of NH3-N, MCP and VFA were determined at Oh, 1h,2h,4h,8h,12h and 24h respectively. The results showed that a decline of pH happened in all groups and pH in groups treated with hainanmycin decreased more; NH3-N concentration increased in all groups, but hainanmycin seemed to inhibit this tendency; MCP concentration in control increased at first then declined later which in other groups altered little; as to VFA, hainanmycin had the tendency to reduce acetate acid concentration and the ratio of (acetate acid+butyrate acid) /propionate acid, and to enhance propionate acid and butyrate acid. However, those results did not represent an obvious effect of hainanmycin. This study indicates that hainanmycin can improve the type of rumen fermentation.2 Effect of hainanmycin on ruminal biohydrogenation in vitroFour adult goats installed with permanent rumen fistulae were used as donators of rumen liquid, and effect of hainanmycin on ruminal biohydrogenation was investigated in vitro. Samples were assigned in 2 groups, including 1 control and 1 group treated with hainanmycin (5mg/L), supplemented with 0.1 mL linoleic acid (50μg/mL) as substrate. The concentrations of C18:2, C16:0, C18:0, c9-C18:1, t11-C18:1 (trans 11-vaccenic acid, TVA), and c9, t11-conjugated linoleic acid (c9, t11-CLA) were determined at 0h, 1h,2h,4h and 8h respectively. The results showed that hainanmycin had the obvious tendency to increase the concentration of TVA and c9, t11-CLA, and TVA was increased significantly (P<0.05) at 1h and 2h; Compared with control, all of other fatty acids concentrations in the group with hainanmycin were obviously higher, except C18:0. C18:2 was increased significantly (P＜0.05) at 2h and C16:0 was increased significantly (P<0.05) at 4h and 8h, while C18:0 was decreased significantly (P<0.05) at 8h. This study indicates that hainanmycin can enhance the concentration of TVA and c9, tll-CLA in rumen, and inhibits the hydrogenation of unsaturated fatty acids.3 Effect of hainanmycin on rumen biohydrogenation in goatsFour adult goats installed with permanent rumen fistulae were used, and effect of hainanmycin on biohydrogenation was investigated in vivo. Goats were assigned in 4×4 Latin square design, fed with colza oil (5mg/goat), hainanmycin (1mg/goat) and colza oil+hainanmycin respectively except the control. The concentrations of C18:2, C16:0, C18:0, c9-C18:1, t11-C18:1 (trans 11-vaccenic acid, TVA), C18:3 and c9, t11-conjugated linoleic acid (c9, t11-CLA) were determined at 4h after feeding. The results showed that hainanmycin and colza oil can both increase the concentration of TVA and c9,t11-CLA, and TVA was increased significantly (P<0.05); Compared with control, the concentration of c9-C18:1, C18:2 and C 18:3 in the group with hainanmycin or colza oil or both were higher, except C16:0 and C18:0, and C18:1 was increased significantly (P＜0.05). This study indicates that hainanmycin and colza oil can both enhance the concentration of TVA and c9, t11-CLA as well as unsaturated fatty acids in rumen.
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