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Salvia Labiatae plant Salvia Salvia miltiorrhiza Bge. Dried roots and rhizomes, bulk herbs, mainly produced in the provinces of Shandong, Henan, Sichuan, Shanxi, Hebei, Jiangsu, Anhui and other. The Huoxuetongluo Stasis analgesic Liangxue consumption carbuncle, pure heart Chufan effects. Previous studies have shown that there is a rich diversity of Salvia in morphological characteristics, chemical characteristics and molecular genetic. A long history of medicinal its Road estate area several transform germplasm exchange is necessarily complex, especially in recent years, With Salvia fierce demand growth, Yaonong introduced from around the Salvia, which is bound to affect the of Salvia quality produce. In order to understand the genetic background of each of the main producing areas of cultivated Salvia, EST-SSR and cpSSR molecular marker analysis of genetic diversity and genetic characteristics of Salvia. First of all, from the United States Center for Biotechnology Information (NCBI) database GeneBank download 10228 Salvia EST sequences, to its SSR information analysis. Discover EST sequences containing SSR loci 1790, accounting for 17.5% of the of Salvia EST sequences Salvia SSR loci of 2014, of which 205 EST sequences containing more than two SSR loci, the Salvia SSR loci frequency of 19.69%. Design EST-SSR primers 104 synthetic 25 for analysis of EST-SSR primer screening. Through literature Search filter out of 20 pairs the universal higher rate cpSSR primer screening primer for cpSSR analysis. In this, the first time established a suitable Salvia EST-SSR, and cpSSR molecular marker technology system. Secondly, selected nine pairs of EST-SSR and 12 pairs cpSSR amplification good, good reproducibility and clear bands SSR primers, the first time the the National 8 provinces and 25 counties in 31 sampling points Salvia EST-SSR, and cpSSR detection analysis. The results show that, Salvia nucleus genetic (EST-SSR) and cytoplasmic (cpSSR), overall are reflected in the medium level. However, in the region there are different degrees of difference. Nuclear genetic diversity based on the Shannon diversity index and Nei's genetic diversity index the provinces range of size as follows: Shandong, Henan, Sichuan, Shanxi, Jiangsu, Anhui, Shaanxi, Hebei; while cytoplasmic genetic diversity provinces between the size of the order : Shanxi, Henan, Shandong, Hebei, Sichuan, Jiangsu, Shaanxi, Anhui. EST-SSR (nucleus genetic) and cpSSR (Cytoplasmic) revealed Salvia 8 provinces range of genetic variation among populations of genetic variation are led. The results show that the limited gene flow between the populations of the provinces of the region, PROVINCES gene exchange. In the nucleus genetic gene exchange between Shandong and Henan provinces, Nm value reached 19.6381; Shandong and Henan provinces have genetic exchange with the rest of the provinces; Sichuan, Shanxi, Jiangsu, Shandong, Henan, Anhui Province gene exchange; limited exchanges between Jiangsu, Shanxi, Hebei, Shaanxi. Significant gene exchange between cytoplasmic Shandong and Henan, Shandong and Sichuan, were 8.8926,8.2068; Jiangsu and Shandong, Sichuan, Henan, Hebei gene exchange exists; Shaanxi, Shandong, Henan gene exchange: Shanxi only with a certain gene exchanges in Henan; limited gene flow between Anhui and the remaining seven provinces. Finally, the through chloroplast genetic analysis that Road estate area and the main producing areas of Salvia place Introduced cultivation, introduction of exotic species source in the cultivation process between Road estate area of ??Sichuan, Shandong, Henan very The long existed germplasm interchangeable; the new Road estate area introduction provenance producing areas. Analysis of genetic diversity of the the National 8 provinces and 25 counties in 31 sampling points Salvia, Salvia exist rich germplasm diversity, provides experimental support for the Salvia varieties Conservation work. Genetic differentiation has not geographical correlation, further extensive gene flow between the national Salvia germplasm is due to human activities caused by offsite introduction of Salvia. Has genetic diversity for the protection of Salvia should try to avoid the large-scale introduction and the off-site, in particular to protect the characteristics of germplasm Road estate area.
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