Dissertation > Excellent graduate degree dissertation topics show

Identification of xrvA Gene Involved in the Virulence of Xanthomonas Oryzae PV. Oryzae

Author: WangChao
Tutor: FengJiaXun
School: Guangxi University
Course: Biochemistry and Molecular Biology
Keywords: Xanthomonas bacteria pathogenic to rice variants xrvA Mutant Pathogenicity Exopolysaccharide
CLC: S435.111.4
Type: Master's thesis
Year: 2006
Downloads: 15
Quote: 0
Read: Download Dissertation

Abstract


Previous work in this laboratory showed that , containing rice Xanthomonas Rice pathogenic variants ( hereinafter referred to as the Xoo ) 13751 DNA recombinant plasmid pGXN3400 of a gene xrvA to regulate bacteria extracellular polysaccharide production of pathogenic and has completed xrvA sequence determination. The xrvA gene is 402 bp, encoding a 134 amino acid - containing product , the product with carpet grass the Xanthomonas bacteria citrus pathogenic variant of the gene (hereinafter referred Xac) xrvA Similar was 98% , the homology is 96 % . In order to understand the role of of rice Xanthomonas Rice the pathogenic variant xrvA gene in the pathogenic process by homologous suicide to integrate plasmid unit point mutations constructed bacteria mutant GXN1280 of . Toxicity testing found in rice by high inoculum concentrations and low vaccination concentration in rice pathogenic than the wild-type strain 13751 , GXN1280 significantly decreased, indicating xrvA gene pathogenicity in rice ; cellular flask fermentation detect extracellular polysaccharide showed that extracellular polysaccharide production is significantly lower than the wild-type strain 13751 . The and xrvA gene of cellulase , xylanase , amylase, protease activity did not affect , xrvA gene regulation DSF factor production . Kinetic experiments by the growth of pathogenic bacteria gene mutant GXN1280 xrvA able to reduce pathogens in plants in vivo pathogenicity and pathogen regardless of the growth of plants in vivo . GXN1280 not affect the growth of the bacteria in the medium . Excess express strains GX03098 plasmid in the growth of rice plants in unstable . Description of the role of bacteria in the pathogenic process xrvA gene may be positively regulates extracellular polysaccharide synthesis work .

Related Dissertations

  1. Functional Analysis of Proteins Encoded by RNA2 of Wheat Yellow Mosaic Virus,S435.121
  2. Study on the Morphology, Physiology and QTLS Mapping of a Dwarf Mutant Ari1327 from Cotton (Gossypium Hirsutum L.),S562
  3. Fine Mapping of Two Leaf Mutant Genes al and yl in Maize,S513
  4. The Effect of Polysaccharide-producing Rhizobium Sp. Q32 on Silicate Minerals Weathering and Its Mechanism,Q93
  5. The Function Analysis of Ceramidase (CER) from Arabidopsis Thaliana,Q943
  6. The Physiological Metabolism of the Curdlan-producing Alcaligenes Faecalis,TS202.3
  7. Relationship between the Mutation of KatG in Mycobacterium Tuberculosis and Drug-resistance of INH,R52
  8. Structure Analysis and Biosynthetic Genes of Exopolysaccharide from Lactobacillus Plantarum C88,TS201.3
  9. Genetic Analysis and Fine-mapping of a Late Heading-date with Dwarfing Mutant in Rice (Oryza Sativa L.),S511
  10. Genetic Analysis and Fine Mapping of Gws Gene Using Green-white-stripe Rice Mutant,S511
  11. Urethra pathogenic E. coli strains U56 virulence gene correlation with in vivo expression differences E516 strain of Avian Escherichia Coli,S852.61
  12. Virulence and Replication of H5N1 Avian Influenza Viruses in Domestic Ducks,S858.32
  13. Isolation and Identification of Pathogenic Canine Distemper Virus and Sequence Analysis of Its H Gene,S852.65
  14. Genetic Analysis and Gene Mapping of Two Mutants in Rice (oryza Sativa L.), Long Glumes Mutant and Narrow Leaf Mutant,S511
  15. Construction of Mutant of Erwinia Carotovora and Cloning of β-mannanase Gene from Bacillus Subtilis and Its Expression,Q78
  16. Genetic Mapping of the Yellow Mutant Gene in Brassica Napus,S565.4
  17. Characterization and Gene Mapping of a Brittle Culm Mutant nbc(t) of Rice,S511
  18. Functional Study on Glycoprotein gG of Bovine Herpesvius Type 5,S852.65
  19. Construction of Mutant Strains and Function of Two-Component Regulatory System QseB/QseC on Actinobacillus Pleuropneumoniae,S852.61
  20. Study on Mutagentic Effects of Space Environment and Biological Changes of Potential Mutants on the SP1 Generation of Dianthus Barbatus L.,S681.5
  21. Identification of Linear B-cell Epitope on Nonstructural Protein 3A of FMDV Serotype Asia1,S858.23

CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Crop pests and diseases and their prevention > Cereal crop pests and diseases > Rice pests and diseases > Disease > Transgression ( pass ) an infectious agent harmful
© 2012 www.DissertationTopic.Net  Mobile