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Nanometer carbon particles having a good lymph targeting, has a strong adsorption at the same time, can be used as a carrier for adsorbing anticancer drugs, to improve the concentration of the drug in the lymphoid tissue, to extend the duration of action, to reduce the drug concentration in blood, and to reduce the systemic adverse reactions etc., is a new drug delivery system for a wide range of development prospects. This article selected cisplatin as a model drug, cisplatin drug-loaded carbon nanoparticles formulations. Nanometer carbon particles used herein, the ball mill grinding prepared nanoscale carbon particles and prepared and chemical methods for comparison. On the particle size, specific surface area, tap density, repose angle, hygroscopic indicators examined. The results show that: the small particle size of the carbon particles obtained by the chemical method, the differential adsorption capacity is not suitable as a drug carrier adsorption; while the smaller particle size of the carbon particles obtained by the ball mill SYSTEM, strong adsorption capacity, suitable for drug loading formulations developed. HPLC determination of in vitro release of free drug content and preparation, separation, quickly and accurately by the method validation, in vitro control applicable to the product. Preparation cisplatin nano carbon suspension of carbon nanoparticles for drug adsorption model. Preparations in vitro release flow chamber was investigated through single factor and orthogonal design, filter out the best prescription, and examine the best prescription vitro release model. Carbon nanoparticles suspension of cisplatin freeze-drying process in order to improve the stability of the drug were discussed, and freeze-dried before and after the suspension of a series of pharmaceutics evaluation, in order to get the optimal prescription and prepared cisplatin nanometer carbon suspension uniform particle size distribution, the average particle diameter of 194 ± 46nm, zeta potential-25.9mv of, pH of 5.62,24 hour cumulative release percentage was 73.62%; the product was freeze-dried to an average particle diameter of 206 ± 51nm, zeta potential-22.8mv, pH 5.55,24 hrs cumulative release percentage was 74.65%, and was left for 3 months at room temperature stability is good. The established biological samples HPLC analysis method cisplatin rats by intraperitoneal injection of cisplatin carbon nanoparticles suspension and control solution, the plasma concentration was measured at different times, the results show that the concentration of carbon nanoparticles preparation in rat plasma lower than that of the control fluid, the drug under the curve area is reduced, the peak time to reduce the systemic toxicity of the drug. This paper examines the drug concentration in rats by intraperitoneal injection of cisplatin carbon nanoparticles suspension and control solution each time point of the heart, liver, spleen, lung, kidney, perigastric lymphatic system, the results show that the preparation in the body of the lymphatic rapid and lasting system in blacking obvious lymphatic targeting and reduced toxicity caused by the accumulation in other tissues. Prove Nano Carbon medicine preparation can improve the target tissue drug concentrations and the ability to change the distribution of drugs in the body tissues.
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