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Serological Proteome Analysis on Trichloroethylene-treated L-02 Liver Cells and the Expression Change of Rho GDIα

Author: XingXiuMei
Tutor: ZhangJianSong
School: Hunan Normal University
Course: Physiology
Keywords: trichloroethylene L-02 liver cells serological proteome analysis Rho GDIα
CLC: R595
Type: Master's thesis
Year: 2008
Downloads: 7
Quote: 0
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Abstract


Objective:①To identify the TCE intoxication related proteins of L-02 liver cells.②To study the Rho GDIαexpression change after the L-02 liver cells were treated with 40μmol/L TCE.Methods:①Three groups of sera were collected which were from healthy donors(control),patients suffering from TCE intoxications and healed ones,which were with serious liver injury.The L-02 liver cells were treated with 40μmol/L TCE,total proteins were extracted and resolved by two-dimensional electrophoresis(2-DE)(pI3-11).Then serological proteome analysis(SERPA)was employed for selection and identification of TCE intoxication related proteins.②Western blot and real time quantitative RT-PCR were employed to detect the protein and mRNA levels of Rho GDIαin L-02 liver cells,which were treated with DMSO and 40μmol/L TCE.β-actin was selected as internal standard.Results:①Six proteins were selected and identified,which were NM23 protein,purine nucleoside phosphorylase,Enoyl Coenzyme A hydratase peroxisoma 1,ribosomal protein P0,lactate dehydrogenase B and proteasome activator subunit 1 isoform 1.②The protein level of Rho GDIαwas significantly up-regulated after the cells were treated with high concentration of TCE,while the mRNA level was down-regulated.Conclusions:①There exist antibodies associated with TCE induced liver injury in patients’ sera.②Six proteins were identified by SERPA which may provided new information for elucidating the underlying mechanism of TCE-induced liver injury.③TCE sitmulation could down regulate the mRNA expression,while the prtotein expression level was up regulated.

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CLC: > Medicine, health > Internal Medicine > Systemic disease > Poisoning and chemical damage
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