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Objective: Research Tian Yuanhuzhitong, particles of autologous intervertebral disc transplantation rat model of lumbar disc herniation of nucleus pulposus phospholipase A2 activity and nerve roots inflammatory cell number, and to investigate the mechanism of action of the inflammatory response in the model of rat nerve root, The study of lumbar disc herniation-induced mechanism of radicular pain, its exact experimental basis for the clinical treatment of lumbar disc herniation. Methods: 72 rats were randomly divided into sham group, model control group, Fenbid groups, the field Yuanhuzhitong particles group, n = 18, the back side of the right side of the waist implanted autologous the caudal vertebrae intervertebral disc nucleus pulposus 5 nerve root method the model group, Fenbid groups, Tian Yuanhuzhitong, particle group rats caused by non-oppressive animal model of lumbar disc herniation, sham group only revealed right L5 nerve root. Rats one day after start giving the drug orally, determination of the five time points rats 1,3,7,14,28 d right foot mechanical stimulation retraction threshold changes, and orally 7 , 14,28 d, respectively, were randomly selected from each group 6 rats were sacrificed and the original transplanted nucleus pulposus (sham group to take the residual the caudal nucleus pulposus) 0 ° C to save. The application trace acid titration detect phospholipase A2 activity, the L5 nerve root biopsy and inflammatory cell count. Results: The model of rat right foot after surgery has obvious mechanical stimulus allergic behavior, and then gradually ease, while the Tian Yuanhuzhitong particle group and Fenbid of rats to mechanical stimulation of the allergy was significantly lower than that in the model group (P lt ; 0.01); group of field Yuanhuzhitong particles and Fenbid group rat nucleus pulposus PLA2 activity and nerve roots inflammatory cell numbers were lower than the model group (P lt; 0.05 or 0.01); Histological observation showed the field Yuanhuzhitong particles group and damage light the Fenbid rats nerve root in the untreated group, 2 groups of nerve fiber bundles arranged a mild disorder, mild edema, fiber bundles to save complete. Conclusion: Tian Yuanhuzhitong, particles can reduce the activity of phospholipase A2 in rat nucleus pulposus, reduce nerve root inflammatory response, promote the recovery of neurological function, and thus significantly alleviate the degree of hyperalgesia rat model of limb, reflecting Tian Hu pain particles analgesic effect in the treatment of lumbar disc herniation in the lumbar disc herniation; phospholipase A2 induced radicular pain to play an important role in the inhibition of phospholipase A2 activity can significantly reduce the inflammatory injury of the nerve root.
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