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Liao Asarum (Asarum heterotropides Fr Schmidt. Var. Mandshuricum (Maxim.) Kitagawa kitag.) The aristolochic Branch (Aristolochiaceae) Asarum genera (Asarum) one of the main varieties of the plant Asarum, mainly distributed in the Northeast region, with the effect of relieving rheumatism and cold, Tongqiao pain, warm the lung of the drink, commonly used in the treatment of cold cold, headache, toothache, stuffy Biyuan and other disease. Liao Asarum mostly concentrated in the volatile oil and quality standards, and the few reports on the study of the chemical composition of aerial parts, in order to further clarify the substance of the Liao Asarum role foundation, Liao Asarum aerial parts chemical The components were studied. Using a variety of chromatographic methods (silica gel column chromatography, polyamide column chromatography, etc.) from the Liao Asarum aerial parts of 95% (v / v) ethanol extracts were isolated 23 compounds by physicochemical properties and spectral data analysis and chemical methods identification of their structure, respectively, for the 3-hydroxy-6-(hydroxymethyl)-8-methoxy-2-methyl-4H-chromen-4-one (1), kaempferol (kaempferol, 2), kaempferol -3-O-β-D-glucopyranoside glycoside (kaempferol-3-O-β-D-glucopyranoside, 3), kaempferol-3-O-rutinoside (kaempferol-3-O-rutinoside, 4) kaempferol -3,7 - two-O-β-D-glucopyranoside glycoside (kaempferol,-3 ,7-di-O-β-D-glucopyranoside, 5), quercetin (quercetin, 6) Quercetin-3-O-beta-D-glucopyranoside glycoside (quercetin-3-O-β-D-glucopyranoside, 7), (10bS) 8,9-dihydroxy-1, 5,6,10 b-tetrahydro -2H-pyrrolo [2,1-a] isoquinolin-3-one (8), niacin (nicotinic acid, 9), indole-3 - carboxylate (1H-indole-3-methyl formate, 10), I (aristololactam aristolochialactam Ⅰ, 11) aristolochic acid IV A (aristolochic acid IVA, 12), p-hydroxybenzoic acid (p-hydroxybenzoic acid, 13), 3,4 - methylenedioxy benzoic acid ( 3,4-methylenedioxy-benzoic acid, 14), homovanillic acid (vanillic acid, 15), 5 - (hydroxymethyl) furoic acid (5-hydroxymethyl furoic acid, 16), succinate (succinic acid, 17), 2,7 - dimethyl-4 - ene - suberic acid (2,7-dimethyl-4-octenedioic acid, 18) (3S) 7,8-dihydroxy-3-methyl-LH-2-benzopyran-4 (3H) -one (19) ,5-oxymaltol (20), 5 - (hydroxymethyl) furfural (5-hydroxymethylfurfural, 21) β-Valley sterols (β-sitosterol, 22), daucosterol (daucosterol, 23). Compound 1 is a new compound, compounds 8-10,14,16,18-20 first isolated from Asarum species are compounds 2,5-7,15 isolated from this plant for the first time. Compounds were obtained and identified by a research group at the same time, Liao Asarum roots Institute of chemical composition are compared, and found that the chemical composition of the Liao Asarum aerial parts and roots have larger differences, mainly: ① aerial parts of flavonoid glycosides Class content higher representative of components such as: kaempferol-3-O-β-D-pyran-glucoside, kaempferol-3-O-rutinoside, etc., and rhizomes of some of these components in the present experimental conditions did not isolated; ② ground and rhizomes portion similar chemical composition as the organic acids, both contents are large, such as hydroxy benzoic acid, succinic acid, etc., and contain a very small amount of aristolochic acid IV A. The above conclusion has been verified by HPLC analysis. Separated according to the various parts of the chemical composition, Liao Asarum ground part of the underground part will play different medicinal effective expansion and utilization of resources, therefore, should be carried out for the Asarum different medicinal parts.
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