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Influence of Magnetic Field on Lipase and Cellulase

Author: YuJingBo
Tutor: JiaShaoYi
School: Tianjin University
Course: Chemical Engineering
Keywords: magnetic field lipase cellulase enzymatic reactions conformation
CLC: Q55
Type: Master's thesis
Year: 2009
Downloads: 44
Quote: 0
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Abstract


In this paper, in order to investigate the influence of the magnetic field on enzyme, lipase and cellulase were studied under the various intensity static magnetic fields which were 0.15T, 0.30T and 0.45T. The enzyme was set in the magnetic field and then the enzyme activity, conformation, enzymatic reactions, activation energy and the influence of pH were investigated. The results showed that there were different effects of magnetic field due to the various enzyme values. For the lipase, the activity was obviously improved by the magnetic field, the value of Km was not very obvious change but Vm was enhanced. The change of activation energy between the magnetically treated and the control was not very obvious. It showed that the reaction rate on the temperature sensitivity was not affected by the magnetic field. The best pH of magnetization was 7 under the experimental condition. The secondary structure of lipase conformation which was detected by the CD(Circular dichroism) was changed after magnetization. For the cellulase, the activity was very obviously improved by the magnetic field; the value of Km and Vm were both improved. The change of activation energy was not very obvious between the magnetically treated and the control. The reaction rate on the temperature sensitivity is not affected by magnetic field. The best pH of magnetization was 4 under the experimental condition. The secondary structure of cellulase conformation which was detected by the CD was changed after magnetization. Besides, the relationship between effects of magnetic field and enzyme was not linear.In this study, the data of experiments were simulated by the Michaelis-Menton Equation, Km and Vm were obtained from the Michaelis-Menton Equation under the various of magnetic induction intensity. We also checked up the results of the simulation.Based on the basic bio-magnetic theory and the results of the experiments, the mechanism of the influence of magnetic field on enzyme was discussed. It was considered that the magnetic field influenced the enzyme by changing the enzyme conformation. The experimental phenomena were explained by this theory.

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CLC: > Biological Sciences > Biochemistry > Enzymes
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