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Base on good properties of glucose and advantages of carbohydrate microarrays technology, the glucose microarrays had a bright prospect. In this paper, first of all, the glucose probes were synthesized by a serious of process which consists of acetylation, bromination, nitro-substitution, amino-substitution and deacetylation. Secondly, MAH-g-Polystyrene was made as the base plate of carbohydrate microarray due to the method which was simple and mature in technology. At last the glucose probes and base plate were coupled with EDC coupling agents.1. The glucose probes were successfully synthesized. Their chemical structure was determined by the NMR and FTIR spectroscopy. Meanwhile, the affection of reaction conditions to productivity of all the process was also studied, and the high productivity would be obtained when chose the below conditions: reaction time, 5.5h; 50mg/g mass ratio of I2 (catalyst) to glucose, amount of dichloromethane: 25 ml/g (glu) and the final yield was 66.53%. The best reaction condition of the second step was obtained as follows: molar ratio of catalysts (TABA) and acetobromoglucose: 1/1, molar ratio of p-nitrophenol and NaOH: 1/1.5, temperature: 90℃, time: 4h, and the final yield was 22.47%. Also, the highest productivity of 1-p-amidophenoxy glucose would be get when reaction conditions as follows: reaction time: 4h, n (reaction substrate) / n (HCl): 1/5, ethanol dosage: 5ml / 0.5g (reaction substrate). When reaction time was 24h, reaction temperature was 25℃and the ratio between deacetylation agents and reactants was 1.5:1, the effection of deacetylation was best.2. The base plates (MAH-g-PS) were successfully synthesized. Their chemical structure was determined by the NMR and FTIR spectroscopy. Also, the affection of reaction conditions to the grafting ratio of procession was studied, and high grafting ratio would be obtained when chose below conditions: mass ratio of the BPO: 1%, mass ratio of MAH: 1%, reaction temperature: 90℃and reaction time was 3h.3. The glucose probes and base plates were successfully coupled with EDC. Their chemical structure was determined by the NMR、FTIR and XPS spectroscopy. The effects of various factors, such as species and the amount of the coupling agent, coupling time and the amount of reaction substrate to coupling effectivity were studied. Also, the NHS was critical to the coupling process.
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