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The Research of Individually Addressable Electroporation Microchips Assisted by Negative Dielectrophoresis for Adherent Cells

Author: YaoHuanFen
Tutor: ChengJing
School: Tsinghua University
Course: Biology
Keywords: Microelectrode Arrays Cell Electroporation Negative Dielectrophoresis Cell Positioning Electroporation Efficiency
CLC: Q819
Type: Master's thesis
Year: 2009
Downloads: 3
Quote: 0
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Abstract


Cell chips and microelectrode arrays (MEA) are very popular and significant topics in current biochip research areas. Cell electroporation (EP) is a technique to transfer genes, proteins and other molecules with a high efficiency, which makes it irreplaceable by other chemical and biological transfection. Compared to many commercial EP products, EP microsystems have various advantages such as low sample consumption, simple power supply, high EP efficiency, and so on, hence they have potential application in the arenas of biomedical research and drug discovery.The objective of this work is to develop an EP microchip composed of MEA and to construct a whole EP system. The combination of negative dielectrophoretic (nDEP) positioning and EP will improve EP efficiency for adherent cells and provide a novel platform for EP research.In this work, we have developed different kinds of EP microchips, discussed the influence of chip designs, cell concentrations and EP parameters, and then selected one optimized chip design and a group of parameters. Finally we have transferred the genes successfully and achieved the individually addressable EP.After the research of nDEP positioning theory, we have developed various nDEP cell positioning microchips, discussed the impact of chip designs and cell concentrations, and then selected one kind of chip designs and the optimized positioning parameters.Based on the research of EP and nDEP, we have further developed novel EP microchips assisted by nDEP positioning for adherent cells. The nDEP force was applied to pre-concentrate the dispersed HeLa cells for directing cells to grow in central EP electrode areas. After the cells adhered in situ, the optimized EP parameters were applied to achieve an efficiency doubled EP, respect to non-nDEP conditions on the same chip. The combination of nDEP positioning and adherent cell EP is a unique feature of this work and lays the foundation for biomedical applications.

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CLC: > Biological Sciences > Bioengineering ( Biotechnology ) > Bio-engineering applications
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