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The Effect and Its Mechanism of PAHs and Surfactants Combined Pollution System on the Growth of Chlorella Vulgaris
Author: XuYin
Tutor: GeFei
School: Xiangtan University
Course: Environmental Engineering
Keywords: Combined pollution Polycyclic aromatic hydrocarbons Surfactant Chlorella
CLC: X173
Type: Master's thesis
Year: 2009
Downloads: 71
Quote: 1
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Abstract
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The composite pollution and its impact mechanism is to encourage research directions in the field of environment, a variety of pollutants interactions and combined effects of great significance to evaluate the actual environmental behavior of pollutants and ecological risk. Polycyclic aromatic hydrocarbons (PAHs), in commenting on the basis of complex pollution system ecotoxicity of surfactants and around the polycyclic aromatic hydrocarbons / surfactant composite Pollution System Center, environmentally sensitive biological - Chlorella vulgaris subjects biological, pollution of PAHs and surfactants single system of Chlorella biomass, to examine the impact of algal cell activity and submicroscopic structure; based on research of the Philippine / hexadecane trimethyl ammonium chloride (Ph / CTAC) and fluoranthene / cetyl trimethyl ammonium chloride (Flur / CTAC) Complex Pollution System chlorella biomass and from the CTAC residual amount, small cruentum absorb nutrients, chlorella Zeta potential preliminary study the mechanisms of polycyclic aromatic hydrocarbons / surfactant complex pollution system chlorella growth. The paper made the following valuable findings: (1) single pollution system of surfactants, surfactant Chlorella biomass inhibition of enzyme activity and submicroscopic structure affect its charge type. Under the experimental conditions, different charge type surfactant inhibition of chlorella: cetyl bromide pyridine (CPB) gt; the CTAC gt; dodecyl sodium sulfate (SDS) gt; dodecylbenzene sulfonate (LAS) gt; Triton X-100 (of Triton X-100), a simple regression calculated by the concentration on the number of probability units CTAC, the CPB, LAS and TritonX-100 96 h-EC50, respectively. 0.18 mg / L, and 0.14 mg / L, 119 mg / L and 341 mg / L; chlorella biomass inhibition rate was 70.7% with 0.3 mg / L under the action of the cationic surfactant CTAC, algal cells appear obvious plasmolysis the protein nuclear distortion and lysosomal expansion; chlorella SOD activity from the control group (6.9 ± 1.0) × 10-6 U / down to (4.4 ± 1.8) × 10-6 U / , ACP relative activity down to 23.1%, the same concentration, anionic surfactants LAS and non-ionic surface active agent Triton X-100 for the chlorella submicrostructure structure and enzyme activity was not obvious. (2) a single pollution of PAHs system, the smaller of polycyclic aromatic hydrocarbons on the the Chlorella biomass and activity. When the concentration of naphthalene (NA), the Philippines (Ph), anthracene (An), fluoranthene (Flur) and pyrene (Py) were 30 mg / L, 1 mg / L and 0.06 mg / L and 0.2 mg / L, 0.1 mg / L, the inhibition rate of chlorella were 25.1%, 5.2%, 4.1%, 6.7% and 10.9%, respectively. NA concentration of 5 mg / L, the SOD activity was 2.3 times that of the control group. The Ph and Flur test within chlorella SOD activity and ACP activity with the control group no significant differences. (3) of PAHs / the CTAC composite pollution system, with the system Ph Flur concentration increased inhibition by the synergistic effect of chlorella biomass into antagonistic effects, synergistic effects showing a decreasing trend after the first increase. Fixed CTAC concentration of 100μg / L, when the the pH and Flur concentration of 5μg / L and 2.5μg / L, synergistic effect reaches the maximum, the maximum inhibition rate was 1.4 times and 2.2 times of the control group; algal cells Zeta potential with forward Ph Flur increase in the concentration of rendering first increases and then decreases, chlorella absorb nitrogen and iron inhibition and solution the CTAC residues showed the opposite trend. Chlorella Zeta potential increases to -10.0 mV from the control group were -9.6 mV and -8.3 mV solution CTAC residues from the control group 54μg / L, respectively, reduced to 45.0μg / L and 41.5μg / L uptake of nitrogen units dropped to 0.095 mg and 0.083 mg, 0.273 mg in the control group were 9.18μg uptake of iron units from the control group were reduced to 3.42μg and 2.14μg.
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CLC: > Environmental science, safety science > The basic theory for the Environment and Science > Environmental Biology > Environmental botany
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