|
Jasmine [Jasminum sambac (L.)Aiton], a evergreen undershrub belonging to Oleaceae family, originated India. Jasmine, one kind of main perfume material, was used at scenting tea, extracting essential oil. Because of the white flower and pleasant fragrance, Jasmine was called the most fragrant flower in world, so it has been widely applied for ornamental at family and courtyard recent years and was deeply liked by people. It is obvious that Jasmine has much more ornamental and economic value. Jasmine has broad prospects on production, development and application. In this article, we discussed the effects basic mediums, auxins, CTks, and temperature on the proliferation in vitro of Jasmine.1. Adventitious buds inducted cultureThe effects of different sterilization time, basic medium (MS、M1、WPM), different concentrations of cytokinins(TDZ,6-BA、KT) and auxins (NAA、IB A) on induction culture of Jasmine were studied. Meanwhile, effects of light intensity and temperature on induction of Jasmine were investigated. The best effect of sterilization was 70% alcohol for 30 seconds,0.1% HgCl2 10 minutes for explant. Results showed that the highest bud induction rate was obtained at the WPM medium supplemented with 2.0 mg/L 6-BA and 0.1 mg/L IBA. Induction rate reached 96.75%, the average number of shoot was 1.97, the shoot was as long as 3.8 cm on average, and proliferation coefficient was up to 2.79. The shoot grew well under light intensity of 6000 lx at 35℃. Shoot cultured from the basal explant had a best stage. Explant grew on May to September was availably for experiment.2. Proliferation cultureThe effects of different basic mediums, carbon sources, on proliferation of Jasmine at different temperature were investigated. Moreover, effects of different concentrations of NAA/IBA and 6-BA/KT, GA3, CH on proliferation were investigated. Results showed that the best basic medium was the WPM medium supplemented with 3% sucrose. The best proliferation with a shoot proliferation coefficient of 5.31 was observed at the WPM medium supplemented with 1.0 mg/L 6-BA and 0.2 mg/L IBA at 35℃. Under the same condition, the average number of shoot was 1.32 and the average length of shoot reached 4.87 cm and the growth was as high as 6.43. However, GA3 and CH played negative role on proliferation. Supplemented with 0.2 mmol/L ferric salt and magnesium salt, Chlorophyll content of shoot was increased. Meanwhile, etiolation symptom of leaf was reduced.3. Rooting cultureThe effects of different medium (MS、1/2MS、WPM and 1/2WPM),auxin (IBA and NAA), rooting culture mode (one-step rooting and two-step rooting), plantlets subcultured in vitro for one to six times and supporting materials (agar, perlite, quartz sand and vermiculite) on Jasminum rooting were studied. The results showed that 1/2WPM optimized rooting for Jasmine. Two-step rooting was the optimal rooting culture mode, with 98.41% of rooting rate. Plantlet was pre-cultured on the rooting medium of 1/2WPM+NAA 1.0 mg/L for 7 to 10 days, then transferring pre-cultured shoots to 1/2WPM without supplement of any plant growth regulators. Rooting ability of the shoots subcultured in vitro 1 to 4 times was well. Besides, the effects of agar, perlite, quartz sand and vermiculite as supporting materials were studied. Vermiculite was the best supporting material; rooting rate was up to 100%; the survive rate of transplant seedlings reached 97.14%; root vigor, POD activity and SOD activity were higher than the other mediums, respectively.
|