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Study on Separation、Purification and Funtion of Antioxidative Peptides from Yellow Mealworm (Tenebrio Molitor L.)

Author: ZhangLiSha
Tutor: ZhangJianXin
School: Northwest University of Science and Technology
Course: Of Food Science
Keywords: Tenebrio Protein hydrolyzate Separation and purification Antioxidant peptide Amino acid composition
CLC: TQ464.7
Type: Master's thesis
Year: 2009
Downloads: 83
Quote: 1
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Abstract


Tenebrio body is rich in protein , the enzymatic hydrolysis of protein preparation of bioactive peptides have been more and more attention and study Tenebrio protein dual protease enzymatic production of antioxidant peptides , and study its biological activity , and for the development of the new antioxidant peptides resources to find an effective way to have an important economic value and social significance . The main results are as follows : ( 1) the degree of hydrolysis and acid soluble peptide yield of indicators identified trypsin and alkaline protease Alcalase optimal enzymes combination and its hydrolysis process optimization . The activity ratio of solid to liquid ratio , enzyme dosage , pH, and temperature factors of enzymatic reaction . Three factors ( enzyme dosage , pH, and temperature ) and three levels of response surface test yield was based on the degree of hydrolysis and acid soluble peptide response value , the best hydrolysis conditions : enzymatic time 120min , enzyme live solid to liquid ratio of 1:1 , 1:3, plus the amount of enzyme 23.41 mg / g , pH 8.77 and hydrolysis temperature 53.4 ° C Up to 21.61% in the optimal conditions for the hydrolysis of low molecular peptide obtained the degree of hydrolysis of the hydrolyzate , acid-soluble peptide yield was 92.09% . (2) through the Sephadex G-25 gel filtration chromatography , enzymatic hydrolyzate is separated into four fractions, the four components of peak 3 of the antioxidant activity is strongest ; use of strongly basic anion exchange chromatography peak 3 are separated into two parts, the antioxidant activity of the component I is higher than the antioxidant activity of the component Ⅱ ; use of strongly acidic cation exchange chromatography component I is separated into two components , wherein the components A to hydroxyl radicals and superoxide radicals Clear strongest clearance rate up to 74.20% and 87.32% respectively . (3 ) Amino acid composition analysis revealed that the component A , glutamic acid (Glu), leucine (Leu ) , alanine (Ala), valine (Val) , the content is very rich, containing eight essential amino acids , representing 41.84% of the of Tenebrio antioxidant peptide amino acids total , the amino acid composition of the reasonable , content -rich , with high nutritional value and a strong health care function . ( 4 ) enzymatic hydrolyzate amino acid color black affect the feel of the hydrolyzate with activated carbon decolorization test , studies have shown that the best decolorization conditions : bleaching pH 3, 3.0% activated carbon , bleaching time of 2h bleaching temperature of 40 ° C in the optimal decolorization conditions decolorization rate can reach 73% or more .

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CLC: > Industrial Technology > Chemical Industry > Pharmaceutical chemical industry > Drug production of biological products > Amino acids,peptides,proteins
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