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Fluoride Impacting on Cytochrome P450 in the Silkworm, Bombyx Mori

Author: LiuZhaoYing
Tutor: ZhuYong
School: Southwestern University
Course: Genetics
Keywords: Silkworm Fluorosis Enduring Fluoride Cytochrome P450 b5
CLC: S884
Type: Master's thesis
Year: 2009
Downloads: 17
Quote: 0
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Abstract


Cytochrome P450 also known as the function oxidases (MFO), catalysis and regulation of its activation and degradation of the metabolism and degradation of endogenous substances or exogenous compounds. Involves the process of growth, development, feeding insects in the role of cytochrome P450 metabolism of xenobiotics characteristics lead to insect resistance to insecticides, also involved in the insect juvenile hormone in vivo, molting endogenous hormones and fatty acids Synthesis and metabolism. The fluoride health silkworm influential, serious died. Bombyx mori fluoride-resistant mechanism is not yet clear, in a lot of work needs to be done on the road explore silkworm fluoride-resistant mechanisms, the study Bombyx mori resistant the fluorine of strain T6 and sensitive strains 734 study materials, different in the fifth instar different periods of the silkworm Tim fluoride treatment after analysis of its cytochrome P450, and fifth instar blood SDS-PAGE electrophoresis pattern, the paper's main findings are as follows: 1. investigation T6 and 734 pairs of fluoride tolerance of T6 and 734 fourth instar fluoride sensitive index during the investigation of the fifth instar use of physiological indicators, mainly to changes in body mass index and mortality, the death body color comparison, assessment of silkworm strains resistant fluorine force proved silkworm T6 strains The highly resistant fluorine ability. 2 the fluoride silkworm cytochrome P450 in the five instar silkworm, T6 and 734 respectively licking different concentrations of fluoride were measured, and then the fifth instar 24h-168h period of cytochrome P450 and b5 content In each of three repeated analysis of variance of the data showed that silkworm T6F2 T6F4 cytochrome P450 content 3-4 times higher than T6DZ, while T6F2, T6F4 difference between the two is not significant, of differences T6DZ significant. Cytochrome P450 content differences between the 734 each treatment, just 734F2, 734F4 P450 content sudden increase in the 24-48h period, and then begin to decline, the sudden increase may stress the role of fluoride. 734F2, 734F4 and T6F2 P450 content was significantly different. T6 in fluoride under increased cytochrome P450 content, while 734 did not change significantly, you can infer the cytochrome P450 fluoride-resistant silkworm synergies may exist. Fluoride-resistant strains the T6 and sensitive strains 734 fifth instar blood proteins by SDS-PAGE on T6 and 734 two strains at different periods of the fifth instar different Tim fluoride treatment of blood protein by SDS-PAGE, the discovery T6F2, T6F4 Tim fluorine the differential bands 168h, standard protein molecular weight 30KD preliminary infer 30KD one or some protein and silkworm Bombyx mori blood fluoride-resistant. For this protein for further study.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Sericulture > Pest and Disease Control of the silkworm
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