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Identification of the Microspore Development Stages and Study of Anther Vitro Culture on Tagetes Erecta.

Author: LiFu
Tutor: TangDaoCheng
School: Qinghai University
Course: Crop Genetics and Breeding
Keywords: Marigold F1 Microspore development Anther culture Hormone combination Hormone
CLC: S681.9
Type: Master's thesis
Year: 2007
Downloads: 23
Quote: 0
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Abstract


June 2005 to March 2007 , taken from the testing ground of the Plateau Flower Research Center of Qinghai University marigold flower buds of the F1 three research ① microspore developmental stages and external morphology of the flower buds ; ② two basic medium , the four hormone combinations , six hormones proportion of anther callus induction rate and callus differentiation rate ; ploidy identification ③ anther culture regenerated plants of different genotypes . The results show that : marigold male gametogenesis can be divided into : meiosis , tetrad , uninucleate , binucleate, nuclear period , pollen six periods ; 's the bracts not yet cracking of inflorescence buds , flowers length between 2.3 ~ 3.0mm , small spores which are in the mononuclear advanced , marigold anther culture sampling time . Anther culture induction rate of callus to MS medium , root differentiation rate of plantlet differentiation rate was significantly higher than B5 ; MS 6-BA/NAA = 20 / 3 is most conducive to callus induction , MS 6 -BA/NAA = 50/3 the most conducive callus redifferentiation . The Callus Induction to reach the peak of the first 3 to 4 weeks after vaccination ; induced peak of 6-BA/NAA combination earliest (16d ) , KT / 2,4- D latest (35d ) . Different genotypes have a significant impact on the anther culture of marigold , four genotypes could be induced callus induction and redifferentiation rate vary greatly . Identified by cytology producer MS basic medium supplemented 6-BA/NAA = 150/3 a marigold haploid , two aneuploid ; MS 6-BA/NAA = 50/3 to get a marigold haploid , an aneuploidy .

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CLC: > Agricultural Sciences > Gardening > Ornamental Horticulture ( flowers and ornamental trees) > Annual and biennial flower class > Other
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