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Studies of Expression of Antioxidant Protein SsPrxQ in Sunflower

Author: LiuYuXin
Tutor: WangZuo
School: Tianjin University
Course: Genetics
Keywords: Antioxidant protein SsPrxQ Sunflower Transgenic Saline-alkali
CLC: S565.5
Type: Master's thesis
Year: 2009
Downloads: 18
Quote: 0
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Abstract


The adversity salinity stress affect crop growth , development and production of one of the main factors in recent years , with the rapid development of molecular biology , transgenic technology has gradually become the focus of salinity research in the field . In this study, antioxidant protein gene (SsPrxQ) by Agrobacterium-mediated transformation into oil sunflower to obtain high salinity resistant plants . Purpose of gene SsPrxQ from Saline Soil Suaeda , and connected to the efficient plant expression vector pBI121 to construct a plant expression vector pBI121 - SsPrxQ recombinant vector plasmids were introduced into Agrobacterium tumefaciens C58 by electroporation , and sunflower infestation transformed . Transformed receptors oil crop oil sunflower , sunflower have been reported in the regeneration system with transgenic this study on the basis of the original system , and further optimization the sunflower hypocotyl differentiation system as well as Agrobacterium genetic transformation conditions , hypocotyl differentiation medium was MS 6-BA0.5mg / L GA30.1mg / L, hypocotyl differentiation rate of 55% ; rooting medium MS NAA0.6mg / L 6g / L agr, rooting rate was 50% ; optimal genetic transformation conditions for the aS concentration 100μmol / L, the bacterium concentration of OD600 value of 0.6 , pre- incubated for 16 hours a dip of 10 minutes , a total of 3 days of culture , culture temperature 24 ° C , Cef concentration of 250mg / L, Kan concentration of 40 mg / L, Gradually filtering through Kan final five resistant seedlings CTAB method transgenic sunflower genomic DNA PCR assay detected three positive the preliminary indication SsPrxQ gene has been successfully imported sunflower cells . The simultaneous extraction Total RNA of transfected gene sunflower done by RT-PCR results show that 3 were amplified specific target fragment , that SsPrxQ gene has been successfully integrated into the genome of the transgenic sunflower and achieve transcription .

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CLC: > Agricultural Sciences > Crop > Economic crops > Oil crops > Sunflower
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