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Biocontrol 153 is trapping separation actinomycetes isolated from soil, The biocontrol strain have a certain potential for the development and application of the test proved. Actinomycetes the 153 inhibitory spectrum is very wide, the Rhizoctonia fungus (Rhizoctonia solani), watermelon (Fusarium oxysporum f. Sp. Niveum), eggplant yellow wilt (Verticillium dahliae) has a prominent sustained inhibition. The research and development of effective viable preparations is the key to be used in agricultural production. Test based on the growth characteristics of the strain 153, optimized liquid - solid two-step fermentation process conditions, screening agents carrier measured synergies and drug-resistant bacteria, aimed at the development and application of actinomycetes viable preparations provide a theoretical basis and technical support. Mycelium as an indicator single factor test and orthogonal design, improved seed medium recipe. The improved medium including: the the sucrose 15g.L -1 glycerol gL -1 , peptone 40 gL -1 0.5 gL KP2PO4, -1 , CaCO3 0.5 gL -1 , NaCl 0.5 gL -1 , MgSO4 05 gL -1 , pH adjusted to 6. Strain 153 via this medium fermented mycelium dry weight increase of 54.1%. Under the growth conditions of strain 153, optimized for solid-state fermentation conditions. Improved solid medium not only increase the number of bacteria, but also enhance the stability of the strains. Its composition was 80% wheat bran, 8% corn meal 10% rice flour and 2% peptone, initial moisture content (0.5% lime-containing water) is 60%. Inoculation conditions for 84h seed liquid, inoculation amount of 20% of the cultivation time was 216h. Under these conditions, the fermentation product of the amount of bacteria increased by 94.1%. Plate culture, heat treatment, and stored at room temperature gradually filtering agents carrier, which chitosan have a prominent role in the protection of 153 actinomycetes. Strain co-culture methods, screening and strain 153 mixed biocontrol strains, including strains 153 and SC11 exhibit good compatibility; prominent synergistic role in disease prevention and FO47 compound processing in watermelon seedlings The role of the root activity, the active area of ??the root surface and root to shoot ratio, respectively, than the blank control 93.5%, 74.5% and 40%. Strain 153 to many common antibiotics and fungicides have a strong tolerance, still containing 2.5 × 103ug.mL -1 streptomycin, rifampicin, penicillin and tetracycline conditions under good growth, but also to adapt containing of 2.5 102 ug.mL -1 procymidone and triadimefon environment. This strain 153 bacterial agents the favorable support combined with control of plant diseases.
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