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Polypyrrole -based electrochemical DNA sensors

Author: LiYuan
Tutor: JiangJiaHuan
School: Chongqing University
Course: Biomedical Engineering
Keywords: Polypyrrole Electrochemical DNA sensor Gene electronics Electrochemical Analysis System
CLC: TP212.3
Type: Master's thesis
Year: 2009
Downloads: 98
Quote: 0
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Abstract


Gene electronics, the term for the first time put forward by the Joseph Wang, etc., used to describe the DNA molecule biometric systems coupled with the electronic interface between systems, with the ultimate aim is to achieve specific recognition of DNA reaction directly into electrical signal, DNA testing , disease diagnosis and ultimately for the development of DNA sensors. Because genes electronics research involves many disciplines, such as surface chemistry, molecular biology, biochemistry and electronics, etc., so the research is full of challenges, but also full of promise. Polypyrrole, one conjugated conductive polymers, and its development as a gene electronics research provided an opportunity. On the one hand, polypyrrole can be used as carrier materials to achieve a flexible fixing probe DNA molecules; hand, polypyrrole can be used as three-dimensional \Based on this study, we use pyrrole, DNA molecular probes of electrochemical polymerization techniques, aiming at the development of a polypyrrole-based electrochemical DNA biosensors, DNA hybridization to achieve the unmarked site testing, as polypyrrole In the electrochemical DNA sensor applications and future gene contribute to the development of electronics. To this end, we made the following several aspects: (1) study the pyrrole oligonucleotide probe molecules formed by electrochemical polymerization of polypyrrole / oligonucleotide (PPy / ODN) complexes Growth mechanism. We found that: In the PPy / ODN aggregation at the beginning, ODN molecules adsorbed on the first electrode by electrostatic particle surface as a nuclear power began polypyrrole polymerization. Subsequently, PPy / ODN nucleation and growth of local reasons progressive nucleation and three dimensional growth mode to an instantaneous nucleation and three dimensional growth mode. After the nuclear particle overlap, PPy / ODN nucleation and growth of three-dimensional nucleation mechanism is instantaneous nucleation and three dimensional growth and progressive growth pattern combination; electrochemical polymerization of PPy / ODN film surface showing folds like structure; ODN molecules can be used as template guide electrochemical polymerization of polypyrrole. (2) the ODN probe molecules as the only anions by electrochemical copolymerization technique ODN probe molecules doped into polypyrrole prepared a molecular network based on cyclic voltammetry of polypyrrole electrochemical DNA sensors. Using atomic force microscopy (AFM) and cyclic voltammetry, we studied and explored prepared electrochemical DNA hybridization detection biosensor mechanism. Summarized as follows: hybridization reaction that PPy / ODN buffer interface with the molecular structure becomes compact and twisted, thus blocking through steric hindrance polypyrrole redox process of cation exchange dynamics. On this basis, the optimal experimental conditions, polypyrrole prepared electrochemical DNA biosensor 5 × 10-18M as low as the detection limit value. (3) the use of 16-bit microcontroller MSP430F169 as the master chip, combined with external analog electronic circuit is designed and implemented based on cyclic voltammetry portable electrochemical analysis system. The system has a battery-powered, simple hardware structure, low power, design upgrades and flexible benefits. With the previously prepared polypyrrole electrochemical DNA sensors can be realized without mark detecting DNA hybridization, detection with good sensitivity. Through subsequent optimization work, the system can replace commercial electrochemical workstation or desktop potentiostat towards DNA testing \(4) combined with microarrays, microfluidics and nano materials, polypyrrole electrochemical DNA biosensor technology trends and application of research to do exploratory prospects. That: By nanomaterials modified electrode and hybridization signal amplification, multi-sample array electrochemical analysis techniques and microfluidic sample manipulation techniques combined with polypyrrole electrochemical DNA biosensors will towards multi-functional, automated, portable, inexpensive DNA hybridization detection system developed for future genetic studies provide a powerful e-learning tool.

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CLC: > Industrial Technology > Automation technology,computer technology > Automation technology and equipment > Automation components,parts > Transmitter ( converter),the sensor > Biological sensors,medical sensors
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