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Effects of Types of Carbon Source on Biological Denitrifying Phosphors and Nitrogen Removal System and Isolation of DPB

Author: YangYongGuang
Tutor: JiFangYing
School: Chongqing University
Course: Environmental Engineering
Keywords: Carbon ERIC-PCR fingerprinting Denitrifying phosphorus removal bacteria Nitrogen and phosphorus removal
CLC: X703
Type: Master's thesis
Year: 2010
Downloads: 353
Quote: 3
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Abstract


Denitrifying phosphorus removal process can be biological phosphorus and nitrogen removal process two originally separate blend is widely regarded as one has good prospects biological phosphorus removal technologies. Thesis by comparing the test, and the use of continuous operation mode of acetic acid, propionic acid, glucose, three kinds of single carbon sources and sewage systems on denitrifying phosphorus removal system, the impact of long-running state to determine the different carbon sources on denitrifying phosphorus removal systems The role and contribution; using ERIC-PCR fingerprinting study analyzed before and after testing each reactor with nitrogen and phosphorus removal flora relationships and characteristics of each reactor flora reveals flora under different carbon sources characterized the evolution of the reactor running state. By denitrifying bacteria isolated with suction / phosphorus release test, and use its preliminary identification 16SrDNA France. The results showed that: ① runs well denitrifying phosphorus removal system that can withstand a short time due to the impact of changes in carbon sources; Three days later, a single carbon source system in the anaerobic phosphorus release and effluent phosphorus concentration fluctuations; two weeks later, acetic acid, propionic acid system gradually restore stability, glucose system will gradually lose the ability denitrifying phosphorus. Acetic acid can improve biological phosphorus removal, can be used as enrichment of denitrifying phosphorus removing bacteria DPB effective carbon; propionate, although it could be improved biological phosphorus removal, but on denitrifying phosphorus removal role in promoting obvious; glucose abound would have become the dominant flora PAOs and DPB gradually being replaced by non-phosphate accumulating bacteria. Acetic acid carbon matrix sludge yield coefficient of less than propionic acid and glucose, glucose carbon matrix is ??conducive for higher sludge yield. ② before and after the test bacteria in each reactor structural analysis showed that: sewage systems and A2N-SBR nitrogen and phosphorus removal system can achieve good stable operation and effect of nitrogen and phosphorus removal, diversity of flora structure showing distribution; single three days before the initial carbon source system is running, the original system is stable complex ecological characteristics proved in a short time be able to withstand the impact of changes in carbon sources. With a single carbon source system operation, the reactor microorganisms in the carbon source in the process of survival of the fittest competition, resulting in the enrichment of the system at the same time with different dominant bacteria, some of the original deposit of microorganisms gradually disappear. Fully proved that in addition to temperature, pH and other factors, the carbon source control system is also an important factor in microbial ecology bit one. ③ DPB denitrifying phosphorus removal systems are an important class of functions flora of BNR play a decisive role. Reported in the literature denitrifying phosphorus bacteria: Enterobacteriaceae (Enterobacteriaceae colonies), Aeromonas (Aerimonas colonies), Pseudomonas (Pseudomonas colonies), Moraxella (Moraxella colonies) and so on. Study found enriched denitrifying phosphorus acid bacteria system has two anaerobic phosphorus release both aerobic phosphorus uptake characteristics typical PAOs and denitrifying bacteria characteristics, numbered P1, P2, P1 aerobic strains phosphorus uptake phosphorus release about three times the amount, P2 strains of aerobic phosphorus uptake of 2 times the amount of phosphorus release and denitrification process from two strains produced nitrite concentration effects. For P1, P2 strain 16SrDNA sequencing results showed that, P1 strain belonging to the genus Staphylococcus, P2 strain belonging to the genus Micro is the existing literature has been reported over the characteristics of denitrifying phosphorus removing bacteria bacterial populations. Showed that denitrifying phosphorus removing bacteria is a broad bacterial groups, biological phosphorus removal system is composed of different species of bacteria work together nitrogen and phosphorus removal capabilities. Research supported by the National Natural Science Foundation of China (50278101) and the State Water Pollution Control and Treatment major science and technology projects (2008ZX07315) funding.

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