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Preparation and Application of Nanoparticles with Fluorescence Molecules

Author: ChenLi
Tutor: ZengZuo
School: South China University of Technology
Course: Materials Science
Keywords: Fluorescence Silica nanoparicles Core-shell structure Ion detection
CLC: TB383.1
Type: Master's thesis
Year: 2010
Downloads: 217
Quote: 1
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Abstract


A large number of experiments have approved that dye doped silica nanoparticles can be used for biotechnology and pharmaceutical materials. The most important thing in nano-biotechnology area is that design materials based on the molecular structure of biological molecules, thereby enhancing the interaction and unification between materials and biological molecule. Actually, the small size and high functionalize is the key to understand the interaction of complex on protein, antibody and cell organ scale, which indicates a breakthrough of problems in the field of biology. Combined the advantages of controllable photophysical properties, different core-shell structures and biocompatibility and so on, dye doped silica nanoparticles is an ideal material in the field of bioscience.In this study, we synthesize silica nanoparticles through sol-gel method, discussed the TEOS hydrolysis mechanism in the formation of silica particles. We study of ammonia, water and TEOS concentration on the impact of nanoparticle size. We make the reaction ofγ-aminopropyl triethoxysilane and NBD-Cl, the outcome hydrolyzed with TEOS to form NBD dye doped silica particles.γ-aminopropyl rhodamine reacte with triethoxysilane isocyanate to form a rhodamine derivatives which contain carbamido and triethoxy, then connect it on silicon surface through the hydrolysis of ethoxytriethyl silane, so that energy transfer occurr between NBD and rhodamine. A probe which use to detect Hg2+ according to fluorescent ratio is produced.This study also explored the Quantum dots/Silica/Rhodamine B energy resonance system. We synthesize high-quality CdTe quantum dots with mercaptoacetic acid as stabilizer, select the TritonX-100 as surfactant, prepared non-toxic water-soluble CdTe-SiO2 core-shell structure composite material using reverse microemulsion method, wrapped a number of quantum dots in the same silica nanoparticle, acquire high-brightness fluorescence SiO2 nanoparticles. We try to attach the rhodamine derivatives on the silicon surface to detect Hg2+.

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