Dissertation > Excellent graduate degree dissertation topics show

A Role of NKT Cells in Immune Disorders After Sepsis

Author: ZhangJian
Tutor: TangChaoZuo
School: Huazhong University of Science and Technology
Course: Trauma surgery
Keywords: Sepsis immune disorders NKT cells
CLC: R459.7
Type: Master's thesis
Year: 2011
Downloads: 24
Quote: 0
Read: Download Dissertation

Abstract


ObjectiveMany studies support the concept that a similar state of immune disorders has been described after numerous forms of sepsis. This state is typified by a loss of delayed-type hypersensitivity(DTH) response and an inability to handle secondary infectious challenges, thus predisposing the patient to an increased chance of developing a nosocomial infection,and multiple organ dysfunction syndrome(MODS)is the most frequent complications and the most common cause of death in most of those patients,so if immune disorders could be early intervened or its course was blocked,it would be a great help for improving the prognosis of sepsis. Most of the present concepts are agreed with that the imbalance of T-cell’s support-suppress network after sepsis caused by many factors for the immune regulation is the core and foundation of different potential mechanisms of the immune suppression after sepsis. NKT cells,as one subset of the Tregs, except for its cytotoxic activity, can act more importantly as a "brake" (especially in the liver tissue) caused by the local inflammation excessive.This study is to explore the roles of NKT cells in the mechanism of immune disorders after sepsis through studing the liver tissue frequency of NKT cells between a mouse model of sepsis and sham mice.MethodsWe selected 8-10 weeks’C57BL / 6 mice, male. Sepsis model were induced by cecal ligation and puncture (CLP) , while sham mice by anesthesia. In order to observe the function of NKT cells in immune disorders after sepsis, the ratio and apoptosis of NKT cells ,and the radio of the secretion of Th1 / Th2 inflammatory factor(IFN-γ/IL-4)of NKT cells were detected by Flow cytometric analysis after separating the liver tissue of sepsis mice and sham mice,Result1. Compared with the sham mice, the ratio of NKT cells in the liver tissue of sepsis mice decreased progressively in the 24 hours after sepsis.2. Compared with the sham mice, the apoptosis of NKT cells in the liver tissue of sepsis mice increased progressively in the 24 hours after sepsis.3. Compared with the sham mice, the secretion of IFN-γand IL-4 increased, and the radio of the IFN-γ/IL-4 increased obviously, indicating that the functions of NKT cells tend to proinflammatory cytokine responsesConclusion1. NKT cells participate in immune disorders after sepsis.2. In early stage of sepsis, the functions of NKT cells tend to Th1 proin?ammatory cytokine responses

Related Dissertations

  1. The Effect of Hemoperfustion in Different Time on the Proinflammatory Cytokines and Survival Time of Sepsis Rabbits,R459.7
  2. The risk Beihai academic thinking and clinical experience and the application of gastrointestinal recovery method of therapeutic effects of sepsis, intestinal dysfunction,R249.2
  3. Multi-center Study of Early Hemofiltration Treat Sepsis Associated AKI,R692.5
  4. Clinical Efficacy Observation with Fu-Zheng-Gu-Ben on Septic Acute Deficiency Syndrome Patients,R278
  5. Septic lung injury (ALI / ARDS) in patients with peripheral immune parameters Th1, Th2, HLA-DR and Treg changes and Integrative Medicine,R459.7
  6. Blood circulation in sepsis induced multiple organ dysfunction syndrome treatment effect,R278
  7. The Significance of Triggering Receptor Expressed on Myeloid Cells-1(TREM-1) in Neonatal Rats Septicemia with E.coli,R363
  8. Effects of Hydrogen Sulfide on the Injury of Colon Mucosa of Septic Rats,R459.7
  9. Application Research of a Novel Septic Rat Model Design by Cecal Ligation and Puncture,R-332
  10. The Effect of Tunicamycin Pretreatment on Septic Neonatal Rat Cardiomyocytes Activity and Mitochondrial Function,R459.7
  11. The Effects of Autophagy on Cardiomyocytes Viability and Mitochondrial Membrane Potential in Sepsis,R459.7
  12. Anti- macrophage migration inhibitory factor monoclonal antibody identification and protective mechanisms Sepsis Study,R459.7
  13. The Influence of Omega 3 Polyunsaturated Fatty Acid on the Immune Function of Sepsis,R459.7
  14. Molecular Epidemiology of Human Parechovirus (HPeV) Infections among Children in Shanghai, China, 2008 Through 2010,R181.3
  15. Correlation between Coagulation Disorders and Sepsis Severity and Prognosis.,R459.7
  16. Clinical Significance of the Dynamic Change of T Lymphocyte Subsets in Patients with Sepsis,R459.7
  17. The Influence of Xuebijing Injection and Low Molecular Weight Heparin on Coagulation Function and Prognosis in the Patients with Severe Sepsis,R459.7
  18. Retrospective Study on the Predictive Value of Th1/Th2 Cytokines in Pediatric Hematology-oncology Patients with Sepsis for the Severity and Treatment,R720.597
  19. Effect of Curcumin on Serum Levels of IL-18 in Rats Sepsis Induced by LPS,R285.5
  20. Sepsis, gastrointestinal dysfunction syndromes Distribution Law,R259
  21. Combined Use Direct Endotoxin Absorption and High Volume Hemofiltration on the Influence of Endothelial Cells,R459.7

CLC: > Medicine, health > Clinical > Therapy > Emergency, first aid
© 2012 www.DissertationTopic.Net  Mobile