Dissertation > Excellent graduate degree dissertation topics show

Effects of 5-Aza-2-deoxycytidine on DNA Methylation and Expression of p16 and MGMT Gene in Lung Cancer Cell Line SPC-A-1

Author: FangHanLin
Tutor: YuZaiCheng;XueShaoLi
School: Anhui Medical University,
Course: Surgery
Keywords: 5-Aza-2’-deoxycytidine p16 gene MGMT gene DNA methylation lung cancer
CLC: R734.2
Type: Master's thesis
Year: 2011
Downloads: 3
Quote: 0
Read: Download Dissertation

Abstract


Objective To investigate the effects of 5-Aza-2 -deoxycytidine ( 5-Aza-dC) on DNA methylation and expression of p16 and MGMT gene in the human lung cancer cell line SPC-A-1 ,and to explore the mechanism of p16 and MGMT gene silencing in human lung cancer cells and the regulating effect of the demethylating agent on p16 and MGMT gene expression.Methods SPC-A-1 cells were cultured in RPMI-1640 medium and were treated with 5μmol/L of DNA methyltransferase inhibitor 5-Aza-dC. Methylation-specific polymerase chain reaction( MSP) was used to detect the promoter methylation state of the p16 and MGMT gene. RT-PCR was used to detect the mRNA expression of p16 and MGMT before and after treatment with 5-Aza-dC, respectively.Results Before treatment with 5-Aza-dC,p16 and MGMT expressions were absent,and promoter hypermethylation of the p16 and MGMT gene were detected in SPC-A-1 cells, After treatment with 5-Aza-dC, the promoter region of the p16 and MGMT gene exhibited a demethylation state, and their mRNA expressions were increased.Conclusion Promoter hypermethylation is a major mechanism of p16 and MGMT gene silencing in human lung cancer cells, and can be reversed by the demethylating agent 5-Aza-dC, which can regulate the expressions of the p16 and MGMT gene.

Related Dissertations

  1. The Clinical Research for the Effect of Iodine-125 Seeds Implanted in Advanced Bronchial Lung Cancer,R734.2
  2. Studies on the Mechanism and Recovery Measures of Hyperhydricity of Pyrus Calleryana Decne in Vitro,S661.2
  3. Methylation of p16 Gene in Plasma and Tissues from Non-small Cell Lung Cancer Patients, Demethylation and the Biological Behavior of Lung Cancer Cell and Transcription of p16 Gene,R734.2
  4. The Clinical Value of Detection of Serum TPS, CEA, CYFRA21-1, NSE for Diagnosis in Patients with Lung Cancer,R734.2
  5. Study on the Expression and Correlation of Metallothionein and Matrix Metalloproteinase-2 in Non-small Cell Lung Cancer,R734.2
  6. The Effect of RNA Interference-mediated ERCC1 Gene on the Chemo-treatment Sensitivity of NSCLC in Vitro,R734.2
  7. Non-small Cell Lung Cancer Analysis of 133 Cases of CVATS Lobectomy,R734.2
  8. Clinical Observation of Pemetrexed for Treating Advanced Non-small Cell Lung Cancer,R734.2
  9. Combined Detection of Tumor Markers in the Diagnosis and Treatment of Lung Cancer to Determine the Clinical Significance,R734.2
  10. Effect of SO2 Exposure on DNA Methylation Polymorphism in Arabiadopsis Thaliana,Q943
  11. Exploreing the Mechanism of Xieshifeiaifang Ii on Treating Lung Cancer with Pleura Effusion,R734.2
  12. The Analysis of the M8000/3 Coder of the Lung Cancer Case in a Hospital Between 2006 to 2010,R734.2
  13. Expression of Chemokine Receptor CCR5 in Human Non-small-cell Lung Cancer,R734.2
  14. Influences of Cisplatin on Expressions of P-glycoprotein in the Resistance Cells of in Lung Cancer Xenografts in the Mice,R734.2
  15. Clinical Research on Detecing of the Combined Survivin mRNA with Cytokeratin 19(ck19) mRNA in Peripheral Blood of Non-small Cell Lung Cancer,R734.2
  16. The in Vitro Study of Serum-free Culture Combined DDP Enriching Human Lung Cancer Stem Cells,R734.2
  17. Effect of Rh-Endostatin on the Expression of ERCC1 in Human Lung Adenocarcinoma A549 Cells,R734.2
  18. The Investigation of Relationship between Therapeutic Effect and Prognosis and Changes of Peripheral Blood Vascular Endothelial Growth Factor and Metallothionein in Patients with Advanced Lung Cancer,R734.2
  19. The Molecular Biology of Rh-endostatin Plus Chemotherapy as Adjuvant Treatment for Non-small Lung Cancer,R734.2
  20. Effectiveness of Erlotinib as the Sennd-line Therapy in Patients with Advanced NSCLC,R734.2

CLC: > Medicine, health > Oncology > Respiratory system tumors > Lung tumors
© 2012 www.DissertationTopic.Net  Mobile