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The Effect of Gambogic Acid on the Growth Activity and Invasion in Renal Cell Carcinoma 786-O Cells

Author: YuanShuai
Tutor: LiHeng
School: Huazhong University of Science and Technology
Course: Surgery
Keywords: GA RCC Cell growth activity Cell invasion
CLC: R737.11
Type: Master's thesis
Year: 2011
Downloads: 38
Quote: 0
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Abstract


Objective: To explore the effect of Gambogic Acid on the growth activity and invasion in renal cell carcinoma 786-O cells.Methods: After treatment with different concentration GA 24h, the cell viability and invasion ability were measured by MTT assay、flow cytometry and invasion assay, respectively. The proteins expression of Bax and MMP-9 was analyzed by western-blot.Results: In comparison with control, the 1.5、3 and 6μM group could inhibit the cell proliferation, and induce the cell apoptosis. The inhibition rate of cell growth were 18.45±3.41%、27.88±6.24%、50.48±12.13% (P<0.05), and the apoptosis rate was21.15±1.68%、33.57±6.35%、46.33±9.74% (P<0.05) respectively. In addition, the number invasion cell was decreased significantly by treatment with 0.375 and 0.75μM group. Moreover, the expression of Bax protein was up-regulated and the MMP-9 was down-regulated, respectively.Conclusion: Gambogic Acid could not only suppress renal cell carcinoma 786-O cell growth but also inhibit the cell invasion. Furthermore, the effect of Gambogic Acid on the cell viability and invasion was association with the expression of Bax and MMP-9.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Urinary tumors > Kidney,renal pelvis tumor
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