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Electroacupuncture analgesia (Electroacupuncture, EA) is a kind of electrical stimulation of modern methods instead of the traditional hand needle reaches the analgesic effect. A large number of studies have shown that electro-acupuncture not only has analgesic effect, and has a two-way adjustment, and to make the animal physiological indicators surgery stable, easier to recover after surgery. Since the 1960s, acupuncture is widely used in animal surgery, anesthesia. Acupuncture analgesia effect of ruminants is much better in other species of animals, the use of acupuncture compound drugs not only overcome the rumen tympany simple drug due to anesthesia, gastric contents reflux problem, and shows excellent analgesia effect, has a good prospect. Small animal (rat) and in vivo studies have shown that central endogenous opioid peptides (enkephalins, p-endorphin, dynorphin peptide) plays a very important role in acupuncture analgesia. Different frequency induced release of various brain regions analgesic substances produce different analgesic effect. Low frequency (2-30 Hz) is the main cause of enkephalin and p-endorphin release, high-frequency (30-100 Hz) induced spinal cord dynorphin release, 2/100 Hz alternating electro-acupuncture induced three the release of opioid peptides. Veterinary practice ruminants acupuncture analgesia appropriate frequency to high frequency (30-100 Hz), frequency (2-15 Hz) above and the suitability of small laboratory animals, acupuncture analgesia superior to other species animals. However, the adjustment mechanism of the ruminants with and small experimental animals, the same is not yet clear. The theory of acupuncture analgesia acupuncture true sense accepted by Western medicine, human medicine and is widely used in clinical practice. Currently, veterinary acupuncture analgesia theory yet rigorous experimental support, has become the bottleneck of China's Veterinary Acupuncture at home and abroad to promote applications. Enkephalin is involved in the regulation of acupuncture analgesia central opioid peptide substances, its release has a frequency-dependent. At present, it is unclear law spectrum of high-frequency electro-acupuncture goats central enkephalin release. The purpose of this experiment is to study the different frequency electro-acupuncture stimulation, high frequency needle in ruminant goat's pain threshold changes and central enkephalin expression levels and distribution law, determine the the best analgesia frequency of goat EA, clarify the central enkephalin thorn analgesia mechanism to provide a theoretical basis for the use and promotion of the veterinary clinic for acupuncture analgesia. In our study, healthy adult 35 hybrid goats weighing 23-27 kg, were randomly divided into five groups (control group, 2 Hz, 40 Hz, 60 Hz and 100 Hz). EA group goat Baihui - tuft of hair on a child A \staining was used to observe the group of goats central enkephalin in the related analgesic nuclei nucleus accumbens, septal area, caudate nucleus, amygdala, supraoptic nucleus, hypothalamic paraventricular nucleus, ventromedial nucleus of the hypothalamus, the brain periaqueductal gray, substantia nigra, dorsal raphe nucleus, parabrachial nucleus, the expression in the locus coeruleus, raphe nucleus and spinal dorsal horn. The potassium ion penetration Determination pain threshold, the results show that the frequency of the electro-acupuncture analgesic effect of 60 Hz goat, goat pain threshold can be increased by 91%, there is a significant difference compared with other EA group (P lt ; 0.05); followed by 40 Hz EA group, able to raise goats pain threshold 69% (P lt; 0.05), a significant difference compared with the 100 Hz, 2 Hz EA group; 100 Hz group is better than 2Hz group, but no significant difference goat pain threshold were increased by 41% and 35% (P gt; 0.05). Enkephalin immunohistochemistry results showed that electroacupuncture frequency of 40 Hz and 60 Hz, enkephalin in the nucleus accumbens, septal area, caudate nucleus, amygdala, periaqueductal gray matter, hypothalamic paraventricular nucleus, under ventromedial hypothalamic nucleus, PAG, substantia nigra and locus coeruleus expression up compared with the control group difference significant (P lt; 0.05); 60 Hz EA group, the nucleus accumbens, septal area, caudate nucleus, amygdala, periaqueductal gray matter, hypothalamic paraventricular nucleus and the locus coeruleus enkephalin expression levels higher than 40 Hz EA group, including the amygdala and periaqueductal gray matter was higher than 40 Hz (P lt; 0.05). The electroacupuncture frequency 100 Hz parabrachial nucleus enkephalin content is higher than the other group, the control group, a significant difference (P lt; 0.05); electroacupuncture frequency of 2 Hz, spinal cord enkephalin highest content with The control group was significantly different (P lt; 0.05). Raphe nucleus and depending on the nuclear EA group enkephalin content is not obvious, no significant differences in the control group (P gt; 0.05). EA raise the pain threshold of the goat, the analgesic effect of 60 Hz goat; enkephalin involved in the regulation of the goat acupuncture analgesia, and the the different nuclei enkephalin release of EA frequency dependence; goat nucleus accumbens, amygdala, septal area, caudate nucleus, hypothalamic paraventricular nucleus, ventromedial nucleus of the hypothalamus, the periaqueductal gray, dorsal raphe nucleus, substantia nigra, parabrachial nucleus, locus coeruleus frequency electric sensitive needles stimulate the release of a large number of enkephalin regulation of acupuncture analgesia spinal dorsal horn release of enkephalin-mediated low-frequency electro-acupuncture analgesia.
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