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Longan (Dimocarpus longan L.) is an important economic value in China famous features fruit trees, but also our very competitive in the international market features fruit, longan size year results, not fruit anniversary of the supply market has not been effectively resolved. these are longan flower bud differentiation. The LFY gene are present in flowering and non-flowering plants, determine floral meristem formation of essential genes and flower formation plays a key role in determining gene series of floral induction pathways output signal and activate floral organ. The LFY gene become a hotspot of genetic research on the floral. Officer Lei (2007) using chromosome walking techniques, clone longan LFY homologous gene coding region of the full-length sequence, and a preliminary study using semi-quantitative RT-PCR technology of LLFY gene function. This test is based on refinement of longan flower bud differentiation sampling process, by semi-quantitative RT-PCR the technology further of LLFY gene expression. Meanwhile, in the Four Seasons honey longan, LLFY expression. In addition, the test application chromosome walking shifting technique cloning the longan LLFY gene promoter sequence fragment, and the promoter sequence for analysis. The main findings are as follows: 1 the longan LLFY expression studies in order to study the LLFY gene expression in different periods of longan flower bud differentiation, the experiments were selected \RT-PCR detection LLFY gene expression. The experimental results show that, the expression of LLFY not only related to the with longan inflorescence differentiation with flower bud differentiation. At the same time, LLFY expression \2 the longan LLFY promoter sequence cloning and analysis using chromosome walking techniques, according to the the longan LLFY coding region genomic DNA sequence specific primers were designed for LA-PCR amplification, to obtain the longan LLFY gene promoter sequence of about 800bp. Plant CARE, FootPriter software for sequence analysis, and the results show that, in addition to containing two major components of the TATA-box and CAAT-box sequence also contains multiple potential cis-acting elements, such as MYB binding sites, ABA-responsive element (ABRE) and light-responsive elements such as GA-box, GAG-motif, I-box, etc., indicating that the longan LLFY expression drought, light, ABA-related.
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