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In this study, adopts ISSR molecular marker mainly on the the in of Cymbidium kanran Makino, Jiangxi Province, the the genetic diversity of of the (Cymbidium kanran Makino) were analyzed, the the purpose of is to understand the the the genetic differentiation among in the level of genetic diversity of the of Cymbidium kanran Makino populations of, genetic structure, as well as populations of, for the cold the the Protection and Development of and utilization of of the Lan resources provide a theoretical basis. Press the the flowering season to points, of Cymbidium kanran Makino is principally is divided into Spring flowering the of Cymbidium kanran Makino type, the summer flowering the of Cymbidium kanran Makino type and the the type three kinds of of the flowering in the fall of Cymbidium kanran Makino. Press leaf size to points, can be divided into the the Types Two of the the the large Ye Hanlan type and the the small Yehan Lan. Therefore, we also, respectively, carried out analysis of of the the genetic differences of the of Cymbidium kanran Makino between the in different types of. The main results are as follows: the establishment of the reaction system and reaction procedures of the suitable for of Cymbidium kanran Makino of the ISSR-PCR. The 25μl PCR is The reaction Dimension was in the, the 1 * PCR buffer, 2.0 mmol / L MgCl2, 100 ng template DNA, 200 ¨ micromol / L dNTP,, 1.4 U of Taq DNA polymerase, 0.4μmol / L Citation matter. Best amplification program was: 94 ℃ pre-degeneration 5 min, and then proceed to the 40 cycles of: 94 ℃ degeneration for 45 s, renaturation may be by the temperature According to the filtered the temperature of the by the of each primer, 45s, 72 ° C for extension of 80 s, the the end of the loop after the 72 ℃ extension 7 min. . Filter out the The 12 ISSR primer pairs Jiangxi Provincial all over the 119 of Cymbidium kanran Makino individual and Wuyishan City, Fujian Province, and Shaowu City, of 75 individuals on, a total of 194 of Cymbidium kanran Makino individual carried out was amplified by, a total of get the 123 bands on, of which Diversity strip band for 97 (Percentage of polymorphic bands for the 78.9%). All the populations of of the flowering the type of of Cymbidium kanran Makino in autumn (including large, lobular), in the the aspects of of genetic diversity, a higher the the genetic diversity of of of Cymbidium kanran Makino in the total the level of on the (PPF = 79.67%, Na = 1.7967, Ne = 1.4461, Hpop = 0.2669, I = 0.3995); level of genetic diversity on the the at population level relatively low to (PPF = 43.97%, Na = 1.4397, Ne = 1.2478, Hpop = 0.1462, I = 0.2204). Of which the highest genetic diversity of the populations of of the Wuyi Mountain in Fujian City, a small Yehan Lan (PPF = 52.85%, Hpop = 0.1732, I = 0.2622). In the genetic structure of aspects of, the the inter-of each populations of there is a higher Levels of Genetic Differentiation, the The coefficient of genetic differentiation (GST) for the the .4719, this the genetic differentiation at which the show that the the the genetic differentiation of in the resided within populations, is is greater than the among populations of the. The results of the analysis of the UPGMA cluster analysis and principal component for also shows autumn flowering the to translate Type of Cymbidium kanran Makino can be divided into into the four branched Department of, branched-Department of The Ⅰ is a the Yifeng (YH), ??Jinggangshan (JGS) and Jing'an (AH) 3 hundred Home group; branched Department of Ⅱ for the-owned Creek (ZX),, big-leaf, Wuyishan City,, and, lobular (WY and WYXH) of the, Wuyishan City,, 3 populations of; the the branched of the branched-Department of Ⅲ for the two of the the the the big-leaf of and lobular of of the Shaowu City, populations of (SW and SWXH); Department of Ⅳ for the Shicheng (SC), Anyuan (GZ-A), as well as the Chongyi big-leaf and lobular (GZ-C, GZXH) 4 UN-base. Of which branched-Department of Ⅰ and Ⅱ poly together, Ⅲ and Ⅳ poly together. This also shows that that the the the the genetic diversity of of the the different mountain range is structure is different, and in Mental inspection results show that the please contact us and we will do our best to make it between the the genetic distance and gene the geographical distance there is no significant correlation (r = 0.3791).. For during different florescence season of Cymbidium kanran Makino Type speaking, the the the coefficient of genetic differentiation in the among populations (GST) for the 0.5231, Description the the genetic differentiation of in the among populations is greater than the the the genetic differentiation of in the resided within populations,. UPGMA cluster analysis and principal component for results of the analysis display a different to flowering the the genetic diversity of of the season of Cymbidium kanran Makino Type populations of there are significant differences. For large Ye Hanlan and small Yehan Lan two type, the the coefficient of genetic differentiation in the among populations (GST) for the 0.0363, the level of gene flow (Nm) for the 13.2655, and greater than 1, Description the the exchange of the gene in the between the these two type is frequent. By on the the large, the small Ye Hanlan the the genetic differences of the are two types of populations of analysis of be drawn the the of genetic variation between the the two as the 7.64%. UPGMA cluster analysis and principal component analysis results also show that large, an insignificant genetic differences in in the the small Yehan Lan among populations. Therefore, we can think that the to from the the on the the level of of evolution members should be belong to the same one kinds of, just the to leaf morphological differ from. For the Wuyishan populations of on the veins segment of Cymbidium kanran Makino speaking, the the the coefficient of genetic differentiation in the among populations (GST) for the 0.3781, Description the the genetic differentiation of in the resided within populations, is greater than the the the genetic differentiation among in the populations of. UPGMA cluster analysis and principal component for results of the analysis display the same Mountains segment populations of an insignificant structural differences in, and in Mental inspection results show that the the correlation of the the between genetic distance and the geographical distance of the was not significant (r = .5854). In addition, the in accordance with the of Cymbidium kanran Makino on Genetic Diversity of distribution pattern of, it is put forward to the a the corresponding protection strategy, such as in situ conservation of,, artificially introduced species are and so on.
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