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Sugarcane mosaic disease is a worldwide disease, the virus genetic engineering has been widely used in sugarcane resistance to mosaic disease breeding. Sugarcane, Ministry of Agriculture, physiological and ecological and genetic improvement of the laboratory in 2005 using gene gun technology will SrMV-P1 gene into the receptor material FN95-1702 obtained six transgenic clones. The study of the turn SrMV-P1 gene material, T3 generation of transgenic sugarcane genetic stability, yield and quality traits and to study changes in active oxygen metabolism and phenolic metabolism virus Stress transgenic sugarcane. The results show that: SrMV-P1 gene can effectively improve sugarcane resistance to mosaic disease ability. Investigation of three generations of transgenic sugarcane field incidence, the transgenic sugarcane incidence less than 5%, the transgenic sugarcane field incidence was significantly lower than the receptor material and no-load P7, which TF64 were highly resistant to three years, are no disease. T3 generation turn SrMV-P1 gene sugarcane SrMV-P1 gene PCR, can still be detected SrMV-P1 gene in transgenic sugarcane, FN95-1702 failed to detect the target gene. Description SrMV-P1 gene in transfected stably inherited gene sugarcane. The T3 generation turn SrMV-P1 gene sugarcane production quality traits were evaluated and the results showed that: transgenic sugarcane growth speed significantly than non-transgenic gene sugarcane faster, turn genes asexual Department TF37, TF50, TF53, TF63, TF64 and TF67 the plant height significantly with higher than transgenic clones the TF50 and control receptor material and no-load P7. On addition to transgenic Clones TF64, other transgenic sugarcane stalk diameter with the receptor material and unloaded controls showed no significant differences. 6 transgenic clones yield than P7, higher than the 5.51% to 43.30%, which TF50, TF53, TF63, TF67 yield higher than the FN95-1702 from 8.22% to 21.38%. Process traits, brix six transgenic lines, turn the brightness cane juice sugar, depending on purity, gravity purity, sugar cane fiber points higher than FN95-1702 from 5.00% to 24.36%, from 10.15% to 36.52% 9.61% to 37.07%, from 4.65% to 9.73%, from 3.29% to 10.25%, from 3.01% to 21.54% and 0.37% to 26.26%. Comprehensive evaluation of the membership function of the fuzzy mathematics can be seen on T1 ~ T3 generation of transgenic sugarcane yield and quality traits TF53 TF64 performance always three generations, FN95-1702 and no-load P7 performance poor. Sucrose acidic invertase gene transfer SrMV-P1 grown sugarcane technology maturity higher activity before help sugarcane nutrition, sucrose neutral invertase activity gradually increased with the advent of process maturity helps sugar cane sugar accumulation. FN95-1702 due to a viral infection, and P7 cane sugar metabolism is more confusing. Turn SrMV-P1 gene disease resistance physiological and biochemical mechanisms that: in four sugarcane mosaic virus inoculation process, transgenic sugarcane TF53, TF64 of H2O2, O2-showed some regularity changes, and late inoculation content at a lower level; the receptor material FN95-1702 and P7 entire vaccination process, H2O2, O2-changes very irregular and its content is maintained at a high level late inoculation. Contrast inoculated before and after MDA content the receptor material FN95-1702 and P7 after virus inoculation the malonaldehyde content than before inoculation were increased by 58.59% and 42.38%, transgenic clones TF53 and TF64 malondialdehyde content no significant increase. Studies have shown that the protective enzymes: The transgenic clones TF53, TF64 after virus inoculation, POD and SOD activity remained at a high level, help to clear excess reactive oxygen species, to protect chloroplast cell structure integrity. Derived phenolic metabolism studies: after virus inoculation stress, transgenic clones TF53 and TF64's PAL, total phenols, flavonoids last are maintained at a high level of resistance to sugarcane actively.
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