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The sugar cane is an important energy herb and the most important sugar crop, sugarcane varieties improved technology contribution rate of 60%, the understanding of the genetic diversity of germplasm resources to help guide crossbreeding parental choice and combination of configuration; understand China sugarcane cultivars and breeding units breeding varieties and advanced lines of genetic similarity of cultivars, area layout and turnover, as well as the follow-up to the relevant units of hybrid breeding program to develop, has important scientific significance and application value. To this end, this study is the first application of the latest international development SCoT mark in 2009, 20 of the 18 bases SCoT primer screening polymorphic primers, PCR reaction system and procedures based on the use of 107 sugarcane clones SCoT mark these sugarcane clones genetic diversity of information, the main conclusions are as follows: (1) the use of 20 of the 18 bases SCoT primer, 107 sugarcane clones were amplified, and a total of 176 bands were amplified , polymorphic bands 163 polymorphism percentage reached 92.85%, eight primers polymorphism rate of 100%, polymorphic information content (PIC), PIC average of between 0.783 to 0.907 0.861 , therefore, has a wealth of polymorphism of that sugarcane SCoT mark. (2) successful genetic diversity analysis of 107 sugarcane clones between sugarcane clones tested genetic similarity coefficient value of 11,449, and the distribution of its value between 0.375 and 0.881, all the test The the sugarcane clones genetic similarity quantitative analysis. (3) the use of cluster analysis and principal component analysis the SCoT marker data of 107 clones tested. In the genetic similarity coefficient of 0.674, 107 sugarcane clones can be divided into six groups: groups Ⅰ mainly distributed Q-, Fujian the sugar and Guangdong sugar Series Guangdong Gan series; group Ⅱ distribution FN series; groups III mainly distributed Yacheng and the part of the CP series; groups IV mainly distributed ROC, CP and India Co series; groups Ⅴ only ROC1 and Q162; taxa of Ⅵ 3 Yuetang and other series clones. The principal component analysis showed that 107 tested clones are divided into domestic germplasm resources and abroad in two categories of germplasm resources, the genetic basis of differences between the two types of germplasm resources, the hybridization of two types of germplasm utilization is expected to significantly improve The hybrid offspring of genetic diversity. (4) the clustering results, to accounting the cultivars of ROC22 more than 80 percent of China's sugar cane acreage ROC16 and ROC10 were distributed in groups Ⅰ, group Ⅱ and group IV, ROC16 and ROC10 the similarity coefficient of only 0.449, and of ROC22 the similarity coefficient of ROC10 also only of 0.455, ROC22 and ROC16 similarity coefficient 0.722, three ROC series cultivars rich genetic diversity, which is beneficial for the security of the sugar cane industry. In addition, the average similarity of the 22 varieties into the national regional trial only 0.593, indicating China since genetic diversity is relatively abundant. (5) in accordance with the breeding units, the 107 sugarcane clones divided into 11 main series of the FN, ROC, Guitang Mintang, Yacheng, Guangdong sugar, Q, CP, and Brazil, India Co., cloud cane and other miscellaneous series, these 12 series and a series of genetic diversity evaluation the 12 series of genetic diversity indices h interval from 0.1635 to 0.3619, miscellaneous series highest (0.3619), ROC (0.3496) CP (0.3466) series, followed by the lowest cloud the cane series (0.1635), shows the difference in genetic diversity of different series. 107 sugarcane clones overall genetic diversity index Ht (0.3656) series between genetic diversity index h (0.3615), 1.01 times and 1.41 times the genetic diversity index Hs (0.2586) within the series, so that the 107 copies of sugarcane clones overall genetic diversity is mainly distributed in the 11 series.
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