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Alzheimer's disease (Alzheimer's disease, AD) is a progressive degeneration of nerve disorders, memory and behavior leading to injury. A promising strategy for the treatment of AD, is the use of acetylcholinesterase inhibitors. Several potent acetylcholinesterase inhibitors, such as of huperzine processing blue he sensitive, originally isolated from herbal to get, which implies that we find potential acetylcholinesterase inhibitors, medicinal plants It is a very rich resource. In our study, in order to find potential acetylcholinesterase inhibitors, anti-acetylcholinesterase activity of 50 kinds of Chinese herbal alkaloid extract by biological self-obvious method, the modified Elman colorimetry combined thin-layer chromatography (TLC) being evaluated. For a significant acetylcholinesterase inhibitory activity of alkaloid extract, they acetylcholinesterase 50% inhibitory concentration determination. The results showed that the Yuan Hu, Treats, Ur, lotus leaf, two needles, purslane, irbesartan mother Fritillaria level, Evodia, Pinellia, Maozhaocao, Radix, aconite with one hundred alkaloid extract shows The significant anti acetylcholinesterase activity, 14 kinds of herbal alkaloid extract of acetylcholinesterase, the 50% inhibitory concentration (IC50) followed 1.93μg/mL 4.9μg/mL 10.8μg/mL, 12.2μg/mL, 17.4μg/mL, 29.4μg/mL, 31.2μg/mL, 50.6μg/mL, 50.7μg/mL, 56.2μg/mL, 59.5μg/mL, 82.9μg/mL, 88.8μg/mL and 102.6μg/mL. The rest of the plant alkaloid extract did not show or shows very weak acetylcholinesterase inhibitory activity. On the basis of the screening results, further separation of the acetylcholinesterase inhibitors nitidum alkaloid extract, 10 compounds. 10 compounds by 1HNMR, 13CNMR, 2D-NMR. EI-MS identification of modern spectroscopy: hair nitidum hormone (1), skimmianine alkali (2), the white moss bases (3), Cliff the pepper alkali (4 ), magnolone (5), plus tin fruit kiosks (6), or Palo level (7), (-) - (S) - plus tin Mi Luo fruit bases (8), 5,6,7 - trimethoxy coumarin (9), Zanthodioline (10). colorimetry through Elman binding modified TLC bioautography 10 compound activity evaluation, the results show skimmianine alkali (LMZ-2) is a needle on both sides the main active ingredient in anti-acetylcholinesterase activity, which shows a moderate activity of acetylcholinesterase inhibition, the IC50 of 33.3μmol / L, and the rest of the compound is not displayed or displayed weak activity. The results of the study suggest that screening acetylcholinesterase inhibitors, traditional Chinese herbal medicine can provide useful lead compounds for the development of drugs to treat AD, acetylcholinesterase inhibitor screening strategy also provides a reference for the screening of other natural inhibitors.
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