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Lobelia (Lobelia L.) plants are mainly located in tropical and subtropical regions, the global total of 380 kinds, China has more than 20 species, mainly produced in the south of the Yangtze River. Lobelia of Campanulaceae (Campanulaceae) lobelia (Lobelia L.) plants lobelia (Lobelia sessilifolia Lamb.),, With roots grass, mainly produced in northwest China's Yunnan Province, northern Guangxi, Zhejiang, Taiwan, Shandong, Hebei, Liaoning, Jilin and Heilongjiang. Chinese Materia Medica \This paper studies the chemical composition of its alkaloids, its system in order to clarify the basis of the effective substances, the use of modern chromatographic and spectroscopic methods. This topic lobelia (Lobelia sessilifolia Lamb.) Grass as the object of study, 5 piperidine alkaloids were isolated by the ultraviolet spectrum (UV), mass spectrometry (HR-ESI-MS and ESI-MS) and nuclear magnetic resonance the modern spectroscopy (1H-NMR, 13C-NMR, HMQC, HMBC) spectroscopy methods and techniques, and its structural elucidation. Two of them are new compounds, respectively named as the new mountain Terrier dishes alkali A and Johor Bahru lobelia alkali B; the remaining three for the first time from this plant for the separation to get 8-ethyl-10-phenyl-norlobelionol, respectively, to the A mountain Terrier ketone alkali and go to the A Cairn Terrier alcohol base. Is looking for a new active ingredient, the four MTT (Methyl-Thiazol-Tetrozolium MTT) reduction screening five compounds were isolated and total alkaloids of lobelia three tumor cell Spc-A1 Hep-G2, MCF-7 growth inhibition activity. The results show that the to go A Cairn Terrier ketone alkali has significant cytotoxic activity on the selection of the three tumor cell lines were significantly inhibited provided having anti-tumor activity of the lead compound for further research and development of lobelia. Alkaloids contained chemical liquid chromatography-mass spectrometry (HPLC-DAD-ESI/MS/MS) total alkaloids in lobelia roots, stems, leaves, flowers qualitative analysis, four different organs component is basically the same, but there are some differences in the relative content of each compound. UV spectra and mass spectra of four different organs of 21 common peaks parse its structure, and Lobelia alkaloids Mass Spectrometry law initially speculated ten alkaloid compound may be a new compound, eleven known compounds, which eleven known compounds are first found in the plants, which provide certain reference for the future of this research is a plant alkaloid chemical composition.
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