Dissertation > Excellent graduate degree dissertation topics show

Study on Purification, Immunomodulating and Antioxidant Activities of Momordica Charantia Polysaccharide

Author: ZhangLiFang
Tutor: HuangWen；ZhangMingWei
School: Huazhong Agricultural University
Course: Of Food Science
Keywords: Bitter gourd polysaccharide Cellulase Ultrasonic wave Separation and purification Immunomodulatory Antioxidant
CLC: R151
Type: Master's thesis
Year: 2010
Downloads: 417
Quote: 3
Read: Download Dissertation

Abstract


Bitter melon is a common vegetable in people's lives, but also the important functional food ingredients. Modern research has shown that bitter melon has improve immunity, lowering blood sugar, antibacterial and antioxidant role, and clearly bitter gourd the polysaccharide (Momordica charantia polysaccharide MCP) is important bioactive components. MCP study at home and abroad are mainly concentrated in the hypoglycemic less of its antioxidant and immune activity and mechanism of polysaccharide sample mostly the unpurified crude polysaccharide, unable to understand the bitter gourd polysaccharide, polysaccharide dose-response between the active ingredient and its active role, structure-activity relationships. Depth study of the MCP in vivo antioxidant and immune activity, antioxidant and immune indicators track the activity of the separated components, structural analysis and access to the active component, aimed at MCP drugs and functionality food developed to provide a certain theoretical basis. The main results are as follows: 1 Optimization of Extraction Process MCP: through the single factor experiment based on secondary rotation regression design experiments., MCP cellulase synergistic ultrasonic extraction conditions were optimized. The results showed that the: MCP optimum process conditions for ultrasonic power 390W, the cellulase of 3500 U / g (enzyme activity ≥ 200 U / mg), solid-liquid ratio 1:38, time 40min, the pH value of 5, the temperature 56 ° C. Under this condition, the bitter gourd polysaccharide extraction rate of 21.1%, than the hot water extraction method, ultrasonic method, the the cellulose enzyme were 7.8%, 13.5% and 7.7%. 2 MCP immunocompromised mice immune activity: spleen lymphocytes obtained from the spleen of normal mice, to take the vitro with polysaccharides culture, way to analyze the impact of MCP on spleen lymphocyte, the results show that: MCP concentration at 60 ~ 100μg/mL range can directly and synergies ConA promote the in vitro proliferation of splenic lymphocytes (P lt; 0.01) and has a two-way adjustment; establish immunocompromised mouse model of cyclophosphamide (CY) modeling method, irrigation the stomach different concentrations of bitter gourd polysaccharides, analysis MCP immunosuppressed mice macrophage phagocytosis, the serum hemolysin content spleen lymphocyte transformation capacity, NK cell activity, spleen index and thymus index of the results showed that: 150mg / ( kg · d), and 300 mg / (kg · d) dose of MCP could significantly improve the immunocompromised mice macrophage phagocytosis, the serum hemolysin content spleen lymphocyte transformation capacity, NK cell activity, spleen index and thymus index (P lt; 0.05), 300 mg / (kg · d) dose The MCP enables immunosuppressive above immunization of mice with normal mice indicators have reached level (P lt; 0.05). 3 MCP's antioxidant activity: Determination of DPPH ·, ABTS radical scavenging capacity and Fe3 reducing ability (FRAP), investigated the antioxidant capacity of the MCP in vitro The results show that: MCP scavenging DPPH and ABTS · IC50 were 571.8 μg / mL, 737μg/mL, concentration in 1.6mg/mL time, MCP DPPH · and ABTS · Clear rate of more than 90%; the MCP of FRAP value 0.187μmolFeSO4/mg, reducing power and solution was a significant positive correlation (P lt; 0.01). Immunocompromised mouse model, the effects of MCP on producing free radicals in vivo activity and free radical levels established by CY: CY spleen XOD the activity of NOS XOD thymus of MPO, NOS activity was significantly increased (P lt; 0.05), serum T-AOC, T-SOD vitality, thymus GSH content significantly with lower (P lt; 0.05); MCP of CY caused by the spleen and thymus XOD, MPO's rising significantly antagonize role (P lt; 0.05), a significant increase in immunocompromised mice T-AOC, T-SOD, GSH levels and decreased MDA content, and make the above test indicators up to normal levels (P lt; 0.05), but no significant effect on NOS activity. Purification and active component of the 4 MCP structure analysis: DEAE-52 ion-exchange column purification of MCP grading, six single polysaccharide component; each component ABTS radical scavenging experiments and spleen lymphocyte proliferation experiment, screening to draw MCP3, MCP6 has good antioxidant and immune activity. These two active components for structural analysis, and the results showed that: the MCP3 and MCP6 are free of proteins and nucleic acid heteropolysaccharide. MCP3 mainly composed of glucose, galactose, arabinose, mannose, xylose, rhamnose, 6 kinds of monosaccharide composition, molecular weight of 2.00 × 104; MCP6 mainly by glucose, mannose, galactose, xylose, arabinose The five kinds of monosaccharide composition, molecular weight of 1.35 × 104. The MCP3 and MCP6 polysaccharide characteristic absorption peaks of the sugar ring of the pyran ring, the MCP3 and MCP6 sugar rings have two kinds of link means, i.e., the glycosidic bond of α-type and β-type glycoside bond. The innovation of this paper is to: (1) the establishment of the bitter gourd polysaccharide cellulase synergistic ultrasonic method efficient method to extract the bitter gourd polysaccharide and conditions; (2) confirmed bitter gourd polysaccharide has strong immunomodulatory and anti-oxidation, (3) 2 isolated from the Momordica charantia polysaccharide having immunomodulatory and antioxidant activity of components identified are low molecular weight hetero polysaccharide, sugar ring is a pyran ring.

Related Dissertations

  1. Study on Antioxidant Components of Tibetan Medicine Sanguo Tang San,R29
  2. The Effects of Abaand H2O2 on sHSPs and Antioxident Defense Enzymes Induced by the Combination of Drought and Heat Stress in Maize,S513
  3. The Difference of Proteome Expression of Low Light Stress and Regular Light Recovery on Growth of Maize Seedling,S513
  4. Applied Research of Hemicell? Feed Additive in Weaned Piglets,S828.9
  5. The Effect of Extreme Temperature on Photosynthetic Physiology, Antioxidant Enzyme Systems and ROS of Fingered Citron,Q945.78
  6. Identification of Marine Sreptomyces Sp. GY-4 and Researches on Its Antimicrobial Substances,Q936
  7. Prokaryotic Expression and Purification of Human Beta-Defensin-9,Q78
  8. Study on Extraction, Isolation and Antioxidant Activity of Lignans of Sesame Cake,TS229
  9. Study on Antioxidant Activities and Lipase-catalyzed Transesterification of Lard to Produce L-ascorbyl Fatty Acid Esters,TS202.3
  10. Isolation, Purification and Antioxidant Activity of Proteins from Chinese Yam,TS255.1
  11. Intrusion detection based on the ultrasonic echo envelope in the military security patrols,E919
  12. Studies on the Effects of Exogenous Nitric Oxide on Rice under Cadmium Stress,S511
  13. Effects of Pre-Anthesis Waterlogging Hardening on Grain Yield and Quality Formation in Wheat under Post-Anthesis Waterlogging and Its Physiological Mechanism,S512.1
  14. The Yunnan ten kinds of pepper antioxidant and antibacterial activity of the research,S641.3
  15. Preparation of Essence and Residues Powder from Flammulina Velutipes and Evaluation of Their Physical-Chemical Properties,S646.15
  16. Effects and Mechanisms of Essential Oils on Quality Maintenance in Postharvest Starawberry and Chinese Bayberry Fruit,S667.6
  17. The Research on the Antioxidant Fat-Solube Compositions of Salicornia Herbacea L.,R284.1
  18. Alleviating Effects of Bacillus Subtilis on the Cucumber under Salt Stress in Substrate Culture,S642.2
  19. Study on Physiological Basis of Seed Aging in Non-Heading Chinese Cabbage,S634.3
  20. Effects of Different Species Meats on Growth, Digestion Metabolism and Antioxidant Status in Rats,S865.12
  21. The Research of the Characterization of Cellulase and Isolation from Omphis fuscidentalis,Q814

CLC: > Medicine, health > Preventive Medicine,Health > Nutrition, hygiene,food hygiene > Nutrition
© 2012 www.DissertationTopic.Net  Mobile