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Origin sectional reddish brown and off-white soil Fuling pharmacognosy

Author: DongKaiXuan
Tutor: DongLiSha
School: Guiyang College of Traditional Chinese Medicine
Course: Of Pharmacy
Keywords: Section reddish brown soil Fuling The sectional class white soil Fuling TLC characteristic map Astilbin HPLC component analysis Kind of the next level of variation
CLC: R282
Type: Master's thesis
Year: 2011
Downloads: 26
Quote: 0
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Abstract


Smilax glabra (Smilacis Glabrae Rhizoma) commonly used herbs in Chinese medicine clinical applications widely recent years, with the deepening of the traditional Chinese medicine research, have been isolated extract more of the chemical composition, and a series of studies of the pharmacodynamic a lot of new theoretical basis for the development of the efficacy and toxicity of new drugs. Part of the \topics intends sectional reddish brown and off-white soil Fuling pharmacognosy and off the contents of a series of studies of the bride glycosides, the active ingredient contained in section-white to reddish-brown soil Fuling whether differences in pharmacognosy and ingredients aimed at finding . This study design ideas: white soil Fuling Section reddish brown and sectional class traits, microscopic identification, intends to find out the difference between the two, whether specificity exists to identify characteristics; 2. TLC identification research the differences, then continue on a different cross-section of soil Poria were established soil of Poria different cross-section colors herbs thin layer characteristic map; 3 of the active ingredients in the soil Fuling section reddish brown and off-white herbs astilbin were HPLC method studies and Smilax glabra herbs for a number of different origin to determine the content. Medicinal species identified by collecting sufficient number of fully representative samples of herbs in a large number of Guizhou and across the country, soil of Poria traits and microscopic identification, identify specificity reddish brown soil Fuling section and section class white herbs identify characteristics, laid the foundation for the next step of the chemical composition and pharmacodynamic study. TLC characteristic map cosine method and correlation coefficient calculated similarity, the two calculation methods calculated similarity basic are greater than 0.95, so the high similarity. The results show that there is a big difference in cross-section reddish brown and the chemical composition between the sectional class white herbs. HPLC determination of soil of Poria Falling bride glycoside content, and the reference to the 2010 version of \acetonitrile -0.1% acetic acid (21:79) as the mobile phase, according to Littleton performed on a Hypersil ODS2 column isolated from each sample peak shape, separation. The final assay results show that the cross-section of reddish brown soil Poria astilbin content ranges from 2.08% to 5.91%, the content of the origin of Guizhou varying degrees of at least 0.45% higher than the 2010 version of the Pharmacopoeia. Astilbin the sectional class white herbs content range of 0.20% -2.42%, content of less than four batches of herbs Pharmacopoeia 2010 edition of the minimum, so there are significant differences in both the content on. Collecting plant samples according to the the above different origin investigation findings to found of Smilax glabra such plants counterpart medicinal roots, some cross-section is almost white, and some section of reddish brown, the two traits, microsurgery, TLC identification The HPLC component analysis and fall bride glycoside content, there are obvious differences. This phenomenon, our preliminary view is that the areas belonging to the population ecology (Population Ecology), the level of variability phenomenon planted.

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