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Evodia for the of Rutaceae plants of Evodia the Euodia rutoecarpa (Juss.), Benth. The stone tiger E.rutaecarpa (Juss.) Benth. Var officinalis (Dode) Huang or sparse the hair the Evodia E.rutaecarpa (Juss.) Benth var. Bodinieri, ( Dode) Huang dried nearly ripe fruit. The Evodia only contained in the \The Evodia the Weixin hot, warm macaroni Yang \For thousands of years, Chinese medicine Xin heat drugs have significant efficacy, the drug is more fierce, normalized by the characteristics of the medicinal properties of the main five internal organs, heart, liver, kidney. Summarizes experimental study in detail on the basis of previous studies, Guizhou Yuqing GAP planting base production thinning hair Evodia as the representative of the spirit of the traditional, simple, easy to reproduce the principles of its temperature through the heart yang efficacy the material basis of the exploratory study. The results are as follows: 1, sparse hair Evodia herbs water, alcohol extraction process optimization. Orthogonal Design sparse the hair Wuzhuyu water extraction, alcohol extraction process conditions, the study shows that: the thinning hair Evodia water extracts prepared optimum conditions: 10 times the amount of water, boiling 3 times, each time fried cook for 120 minutes, the optimum conditions for the sparse hair Evodia ethanol extract prepared: six times the amount of 60% ethanol extracted three times, each time 120 minutes. By the HPLC determination contrast, TLC Pattern Method HPLC characteristic spectrum comparison method, investigation of sparse hair Wuzhuyu water extract ethanol extract of dried method, the results show that: the vacuum drying, microwave drying, freeze drying the extract quality no statistically significant impact from energy-saving, less consumption, save time, according to the actual situation in many ways, the appropriate choice of the method of drying. 2, sparse hair Evodia water, alcohol extract HPLC characteristic spectrum. System of the Evodia water, alcohol extract HPLC characteristic spectrum, Evodia extract characteristic spectra: column is ZORBAX Eclipse XDB-C18 (4.6 × 150 mm, 5 μm), mobile phase of acetonitrile -0.1% formic acid water, using a mobile phase gradient elution system (time: 0 min to 5min ~~ of 38 min ~ 65min, acetonitrile ratio: 5% ~ 5% ~ 23% to 100%), a flow rate of 1 mL · min-1, detection wavelength 258nm, column temperature was 30 ℃. The fingerprinting method by methodological study, precision, stability, repeatability, marked 20 characteristic peaks Evodia alcohol extract and water extract 19 peaks. 3, LC-MS technology of thinning hair Evodia extract biometric spectrum peaks qualitative analysis. Electrospray ionization - tandem mass spectrometry manner established the UPLC-MS/MS analysis method in the existing literature on the basis of the reference evodiamine compound contained in the chromatographic behavior of cleavage and mass law, by optimizing binary gradient elution and MS performance parameters, identification wherein eight of active ingredient, respectively, chlorogenic acid and its isomers, quercetin-3-0-β-D galactosidase, dehydroepiandrosterone Evodia alkali, limonin prime, 14 - formyl dihydro rutaecarpine, evodiamine and rutaecarpine. Thinning hair Evodia extract the main active ingredient, rich feature maps reflect the chemical information, and also provide a basis for better clarify the material basis and mechanism of action of traditional Chinese medicines in liquid chromatography-mass spectrometry can be quickly and accurately identified. 4, the thinning hair Wuzhuyu water, chemical composition and biological activity of the alcohol extract relatively characteristic spectrum of drugs and temperature through the heart yang comparison. The sparse hair the Evodia water, alcohol extract the total alkaloids transfer rate is 5.45% and 71.22%, respectively; sparse hair Evodia water extract, ethanol extract and herbs HPLC characteristic spectrum comparison, water, alcohol extract feature maps and herbs feature maps similarity of 0.545% and 0.933%, respectively, and both results showed that the alcohol extract more water extract retained for the chemical composition of the herbs. Water extract, ethanol extract HPLC feature map comparison, we found that there are certain differences in the peak area of ??extract fractions of different polarity. Through three \Further the efficacy of screening experiments also showed that the alcohol extract for the role of left ventricular pressure and heart rate greater than the water extract, confirmed the quality of the alcohol extract than water extract, sparse hair Evodia laid the foundation for the further positioning of parts, but also provide a strong reference value for clinical.
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