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Variance Analysis of Endogenous Cytokinin and Auxin in Different Phyllotaxis Plants

Author: LiuTianBao
Tutor: ChengBeiJiu
School: Anhui Agricultural University
Course: Biophysics
Keywords: Phyllotaxy Apical meristem tissue ( SAM ) Auxin (IAA) Cytokinin (CTK) CTK / IAA values
CLC: Q944
Type: Master's thesis
Year: 2010
Downloads: 109
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Abstract

Phyllotaxis phenomenon, plant leaves around the stem are regularly arranged three common in nature phyllotaxis types are: alternate, raw and whorls. The study showed that the apical meristem (SAM), cytokinin (CTK) and auxin (IAA) is involved in the formation process of the phyllotaxis. Auxin transportation and differences in the meristem at the top of the distribution to control the formation of leaf primordia cytokinin affect the size of the SAM and auxin transport protein gene expression affect the distribution of auxin in the SAM indirectly affect the formation of phyllotaxis while apical meristem CTK / IAA stability and change of field values, may also be an important factor in regulating the stability and variability of phyllotaxis. In this study, using enzyme-linked immunosorbent assay (ELISA) determination of cytokinin and auxin content in a variety of different phyllotaxis plant apical meristem and around the part of the plant apical meristem young stems and leaves of cytokinin and auxin content analysis of different phyllotaxis plant endogenous cytokinin and auxin differences, obtained the following main results: 1. analysis showed that the hormone content in different growth period of the test material in the apical meristem, the top of the different growth stages of different phyllotaxis plants was no significant difference in the meristem cytokinin and auxin content and CTK / IAA values. Cytokinin and auxin content of its surrounding young stems and leaves differences in the apical meristem obvious rule, but the apical meristem CTK / IAA values ??significantly higher than the surrounding young stems and young leaves. 3 different plants phyllotaxis plants apical meristem hormone content and CTK / IAA values ??follow the law. There are significant differences in the various types of hormone content and CTK / IAA values ??phyllotaxis plant apical meristem. Apocynaceae the oleander top of whorled phyllotaxis meristem organization cytokinin content and CTK / IAA values ??significantly higher than phyllotaxis Changchun spent three categories cytokinin content Cytokinin Contents CTK / IAA values ??were significantly higher; Asteraceae, meristematic phyllotaxis marigold top organization significantly higher than the alternate phyllotaxis chrysanthemum, three categories were significantly higher cytokinin . Jerusalem artichoke and snapdragons show that the same plant phyllotaxis plants, old plants apical meristem, cytokinin content and CTK / IAA values ??were significantly higher than the alternate plants, and three categories cytokinin not less than the alternate plants; auxin content was no significant difference on top of the old plants with alternate plants meristem. In summary, the close link between hormone content and ratio phyllotaxis type apical meristem. Within the same family or closer genetic relationship, whorled plant apical meristem The cytokinin content CTK / IAA values ??are significantly higher than plants on the top of the plant meristem cytokinin content and CTK / IAA values ??were significantly higher than the alternate plant trend. Apical meristem, cytokinin content and CTK / IAA values ??may adjust the the leaf sequence variation important factors.

Variance Analysis of Endogenous Cytokinin and Auxin in Different Phyllotaxis Plants

Abstract

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