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The Capillary Electrophoresis Fingerprint of Holstein Bovine Milk Proteins and Its Application

Author: ShiYan
Tutor: LiuNing
School: Northeast Agricultural University
Course: Of Food Science
Keywords: Capillary zone electrophoresis Fingerprinting Milk protein Milk adulteration Mixed milk
CLC: TS252.7
Type: Master's thesis
Year: 2010
Downloads: 70
Quote: 1
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Abstract

In recent years, China's raw milk adulteration behavior common to obtain high profits, some unscrupulous manufacturers to improve the protein content in the raw milk to impersonate or substitute milk protein mixed with cheap milk protein and non-protein nitrogen, fraud consumers, even hazardous to their health. In order to stop this phenomenon, at present, there is an urgent need for a rapid detection method to detect the protein components of milk, to detect adulteration of raw milk. The purpose of this project is to use capillary electrophoresis raw milk protein ingredients, to establish milk protein fingerprints detected in raw milk adulteration. The main subject of research from the following four aspects. First, the application of the uncoated capillary zone electrophoresis and the coated capillary zone electrophoresis are two ways to detect the protein in milk, choose one of the more suitable detection method as a separation method for the determination of milk protein. And the selection method of the optimization of the electrophoresis conditions. Five milk protein measured electrophoresis method with optimized standard products, raw milk accurate qualitative and quantitative analysis. Second, the application of the selected method to establish Holstein legislation cow's milk protein fingerprints, evaluate the pros and cons of fingerprinting from the similarity of content similarity and fingerprint index. Third, the application capillary electrophoresis fingerprint testing of raw milk mixed with non-protein nitrogen situation. Fourth, the application select optimized capillary electrophoresis method to detect mixed milk, milk protein distribution, the qualitative and quantitative detection of mixed milk adulteration. The results are as follows: the experiment fully proved two experimental methods for capillary zone electrophoresis, respectively, from the separation, the qualitative and quantitative characteristics, separation conditions and methods of inspection and other aspects result of the comparison shows that the coated capillary superior uncoated capillary Methods. And method of coating capillary electrophoresis conditions were optimized. 2. Applications optimized coating capillary zone electrophoresis method analysis of 10 Holstein raw milk origin of milk protein, to determine the 10 milk protein peaks can be used for the qualitative identification. Reproducibility, stability, and better recovery results, to meet the requirements of the quantitative detection. Holstein milk samples of different batches of fingerprint comparison, fingerprint similarity, content similarity, with good results to evaluate the pros and cons of the fingerprint, the other with the chromatographic fingerprint index F and the relative index Fr, so as to establish a raw milk protein capillary electrophoresis fingerprints. Establish raw milk protein fingerprinting to detect the case of non-protein nitrogen added to the milk, add non-protein nitrogen experiment as analog, qualitative judgment raw milk adulteration. 4 applications optimized coating capillary zone electrophoresis method to detect milk mixed milk mixed with goat milk adulteration. To identify the characteristics of casein could clearly distinguish two milk by capillary zone electrophoresis diagram comparing different species milk, based on the difference of the the various casein migration time and peak area ratio and add the amount of linear relationship to the characteristics of casein Quantitative analysis of the adulteration of milk. The subjects using high performance capillary electrophoresis analysis of milk protein ingredients, electrophoresis optimized conditions: polyacrylamide coated capillary column, separation voltage 25 KV, pH = 3.0, column temperature at 25 ° C, 214 nm detection wavelength; This method measured five standard protein in the milk within the linear range of linear correlation coefficient are 0.9986; capillary electrophoresis fingerprints, the similarity in the determination of 10 raw milk samples above 0.950; and determination of milk and goat's milk mixture casein peak area ratio of the mixing ratio was a good linear relation, the linear correlation coefficient of 0.997 or more, can be detected as a quantitative indicators of the mixed milk.

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