|
Before ovulation, the stimulation of endogenous gonadotropin peak compaction around around the oocyte cumulus cell expansion occurs, a phenomenon known as cumulus expansion or mucus. Cumulus expansion has important implications for ovulation and fertilization process. Previous research has shown that there is a way to suppress cumulus expansion factor follicular fluid. In this paper, the goat follicle CEIF (cumulus expansion inhibiting factors) the presence and source. We use interspecific test systems, applications mice, removal of oocytes cumulus oocyte complexes (oocytecomized complex, OOXs), detection goats, follicular fluid and follicles in various body cells secrete CEEF. Mouse COCs detected body in a variety of goats, follicular fluid and follicle cells secrete the CEIF the situation, and try to determine CEIF what kind of substance. We also tested the the four amino dextran goat COCs cumulus expansion in mice of COCs role. The results are as follows: 1, 10%, 50% in the follicular fluid of the mouse OOXs 55-60% extension That the goat follicular fluid CEEF; 100% in follicular fluid can be suppressed mice COCs expansion, in the follicular fluid containing CEIF. 2, the cultured mouse OOXs in various concentrations in follicular fluid are not extended, large follicular fluid does not contain CEEF; various concentrations of large follicular fluid cultured mouse COCs are extended, indicating a large goat follicular fluid does not contain CEIF. 3 cultured mouse OOXs of the follicular fluid of the various concentrations of PG 24 hours after no extension, follicular fluid containing no CEEF; same mice treated COCs can expansion occurs, indicating that this PG 24 hours after follicle solution does not contain CEIF. 4, the cultured mouse OOXs in follicular fluid the various LH after 20 hours taken are able to expand, and most OOXs are four expansion LH 20 hours after taken the follicular fluid contains lot of mouse COCs the CEEF; same treatment can be extended, and the vast majority of four expansion LH 20 hours after taken follicular fluid does not contain CEIF. 5, the cumulus cells and the wall of the granulosa cell conditioned medium CEEF activity, rather than with CEIF activity. With different the cumulus cell wall granulosa cells, cell membrane conditioned enables the mice the COCs's expansion 20% inhibition the membrane cell conditioned medium both CEIF activity also has CEEF activity. The CEIF likely from the membrane cells in follicular fluid. Goat follicular fluid (2 ~ 4mm), follicular fluid (5 to 6mm), 24 hours after the body PG taken follicular fluid content of glycosaminoglycans in follicular fluid in the 20 hours after the LH peak followed by 1.2 mg / ml, 0.91 mg / ml, 0.6 mg / ml, 0.7mg/ml. Cumulus cell conditioned the membrane cell conditions of glycosaminoglycan content of 0.14 mg / ml, 0.12mg/ml. 7, amino-dextran (Hep, CS-B, HS, CS-C) in order for the murine COCs extension inhibitory concentration is 0.01mg/ml, 0.1mg/ml, 1mg/ml, 5mg/ml. 8, the aminodextran (Hep, CS-B, HS, CS-C) are not inhibit the the goat cumulus expansion of COCs. 9 follicular fluid can be suppressed, goats goat cumulus expansion of COCs. 10 follicles, follicular fluid of large follicles stearic acid (C18: 0), oleic acid (C18: 1), linoleic acid (C18: 2) is the major fatty acid, linoleic acid content in follicular fluid were significantly higher than Big follicular fluid. In summary, goat follicular fluid the CEIF activity, and is mainly secreted by the membrane cell. For goats COC, amino dextran is not inhibited. Glycosaminoglycan goat follicular fluid, cumulus expansion is not the main inhibitory factor. Follicles linoleic acid may be the main cumulus expansion inhibitory factor.
|