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Gosling plague (Gosling plague, GP) by Parvoviridae dependent Goose parvovirus (Goose Parvovirus, GPV) virus, the cause of a deadly infectious diseases, mainly against 4-day-old to 20-day-old goslings and Muscovy Duckling, caused acute enteritis and liver, kidney, heart, and parenchymal organs septic lesions, particularly in the fiber of the small intestine, embolization venereal becomes the main feature, morbidity and mortality can be as high as 90%. Currently, the disease every year, a different degree of popular, one of the most important infectious disease of geese and Muscovy duck areas, causing serious economic losses. Because of the disease has long been plagued by goose industry development, the need for an accurate, simple and rapid diagnostic method to make an early diagnosis of the disease. In many areas since the colloidal gold immunochromatography (GICA) technology is a simple and rapid immunological detection technology since the early 1990s by Beggs, etc. used in the determination of human chorionic gonadotropin (HCG) There are applications, such as the detection of hormones or disease related protein; of pathogenic microorganisms protein or antigen antibody detection; parasites associated antigen, the detection of antibodies; blood, urine drug concentration monitoring. Still not applied this technology detects goose parvovirus antibodies reported, due to the characteristics of this technology will be applied to the rapid detection of goose parvovirus antibodies, will goose the healthy development of the industry have a major impact. GPV-VP3 protein is the major capsid protein of the virus, accounting for about 80% of the total content of the capsid protein, the amino acid sequence of exposure to the surface of the virus particles are present in the VP3 protein within the polypeptide chain, and VP3 containing GPV major antigenic epitope ingredient GPV primary immune protective antigen, goose serum antibody capable of specifically binding to natural infection, and therefore, which can be used as a gold-labeled antigen, the capture antibody. Sodium citrate - gold chloride acid oxidation reduction prepared 18nm colloidal gold particles mark Goose parvovirus (GPV) VP3 recombinant protein produced gold standard pad test line of capture antibody rabbit anti-goose IgY polyclonal antibody, QC line capture antibody rabbit anti-VP3 polyclonal antibody, assembled colloidal gold immunochromatographic strip detection of goose parvovirus antibody, and Point-of-care testing (POCT) scanner quantitative research, the minimum detectable amount of 9.89 ng / mL. The obtained test strip with the agar diffusion test (AGP), compared to the enzyme-linked immunosorbent assay (ELISA), in line with the rate of 100%, 97.7%. In this study, the test strip specificity, high sensitivity, simple, rapid, repeatable, so it can be used for the rapid diagnosis of goose parvovirus antibody, the monitoring the goose body's antibody levels and its variation, to facilitate timely immune protection, reduce the harm of the disease on the goose industry, and is conducive to the the goose industry healthy and stable development.
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