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Liriodendron hybrids is a rare species and ornamental landscapes important industrial timber species. However, due to the low seed natural species, reproductive ability, hybrids in short supply. Through the establishment of the vegetative organs of hybrid Liriodendron somatic embryogenesis system for solving Liriodendron seedlings to provide an inadequate supply of new ways. In this paper, hybrid Liriodendron vegetative organs of materials, including biennial winter branches, twigs and annual young leaves, petioles, etc., to totipotency of plant cells as a theoretical basis, based on the existing hybrid Liriodendron immature embryos somatic embryos generating system as a guide for the vegetative organs of somatic embryogenesis. Analyze and understand the vegetative organs genotype, medium hormones, culture conditions, osmotic pressure on somatic embryogenesis. The results showed that: in 3/4MS basic medium, add 2.0mg / L concentrations of 2,4-D, 0.3mg / L of 6-BA better callus, respectively, KT concentration of 0.5mg / L, IAA concentration of 0.2 mg / L ,6-BA concentration of 0.5 mg / L and ZT concentration of 4.0 mg / L, IAA concentration of 1.0 mg / L, can induce relatively compact embryogenic callus. In the process of induction of somatic embryogenesis, ABA can give embryo growth creates an environmental stress, thereby inhibiting the production of abnormal embryos. Lighting conditions are somatic embryos produced late and body seedling essential factor. Liriodendron by hybridization liquid suspension culture in the solid culture system based on the study of somatic embryogenesis for further study. Experiments to study the impact of speed on callus, drawn 95 r / min for the best speed. Studied in suspension culture callus proliferation process to arrive at the ideal time for the 25 days of subculture. While the suspension lines were induced to obtain a globular embryo for somatic embryogenesis in vegetative organs conducted a feasibility study to explore. Through the hybrid Liriodendron vegetative organs during induction of somatic embryogenesis and somatic embryogenesis immature embryo induction process, each stage of physiological and biochemical research, from the horizontal and vertical are compared and analyzed. The results show that the test material with the number two lines NE04 and NE55 number of soluble protein and soluble sugar content is high, five in the selected genotypes, having a relatively strong ability of somatic embryogenesis, but obtained in this experiment The results show that the ability of somatic embryogenesis and other indicators of immature somatic embryos far and the results obtained. Use of mature somatic embryogenesis organs need further exploration.
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