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This paper examines the ketoprofen enantiomers polysaccharide chiral column (Chiralpak AD column , Chiralcel OJ column , Chiralpak the AS column and Chiralcel OD column) on the chromatographic behavior as well as mobile phase composition on the separation of enantiomers , the establishment of the method for separation analysis of ketoprofen enantiomer in plasma samples , and for determination of ketoprofen enantiomer . Chiralpak AD (10μm, 250mm × 4.6mm id Daicel Chemicals) column with n-hexane - isopropanol - TFA ( 80:20:0.02 , v / v / v ) as the mobile phase , detection wavelength 254 nm, flow rate of 1.0 ml / min, column temperature 25 ° C as the chromatographic conditions in the analysis of plasma samples was separated ketoprofen enantiomer , results ketoprofen enantiomers within 10 min to achieve separation, the separation is greater than 2.0 , the enantiomer R and S , respectively, in the blood concentration of 0.1μg/ml - 10μg/ml range a good linear relationship related factors were 0.9998 and 0.9996 , the lowest detection limit of a single enantiomer are 2 ng ( S / N = 3 ) . The method is simple, high sensitivity , accuracy , and can be used for the detection of single optical purity of enantiomers and pharmacokinetic study .
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