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Objective: To investigate TGF-β1/Smads pathway in airway remodeling in asthmatic rats model of the dynamics of its role in the pathogenesis of asthma, research Cai Pu Tang treatment of bronchial asthma possible mechanism. Methods: 70 healthy male SD rats were randomly divided into group A, 10, B group 60 is divided into (asthma two weeks B1 group 10, group B2 10 asthmatic four weeks, eight weeks B3 asthma group 10, Cai Pu Tang two weeks group B4 10, Cai Pu Tang four weeks B5 group 10, Cai Pu Tang eight weeks B6 group 10). Copied atomized with ovalbumin asthma model in asthmatic two weeks, four weeks and eight weeks later, with the reverse transcriptase polymerase chain reaction (RT-PCR) detection of airway TGF-β1, α-SMA mRNA levels, with the Immunohistochemical staining of airway TGF-β1, α-SMA, Smad3, Smad4, Smad7 protein expression, while using image analysis measurement of airway morphological parameters. Results: 1. Airway remodeling in asthmatic rats changes: asthmatic rats with excitation time, airway remodeling is more obvious. Asthmatic rats two weeks after excitation seen around the bronchial wall inflammatory cell infiltration, airway smooth muscle thickening, goblet cell hyperplasia and mucus hypersecretion, excessive collagen deposition, basement membrane thickening; asthma four weeks group compared with two weeks , airway smooth muscle thickening, airway wall is thicker, the difference was statistically significant (P lt; 0.05); asthma eight weeks group compared with the four weeks of airway smooth muscle thickening, luminal narrower, the difference was significant sex (P lt; 0.05). 2 asthmatic rats TGF-β1, α-SMA, Smad3, Smad4, Smad7 protein expression and TGF-β1, α-SMA mRNA levels: asthmatic rats two weeks after excitation airway TGF-β1, α-SMA , Smad3, Smad4 protein expression and TGF-β1, α-SMA mRNA expression levels, and decreased expression of Smad7, four weeks after the TGF-β1, α-SMA, Smad3, Smad4 protein expression and TGF-β1, α-SMA mRNA levels increased further, while the expression of Smad7 further decline, compared with two weeks, the difference was statistically significant (P lt; 0.05); eight weeks after TGF-β1, α-SMA , Smad3, Smad4 protein expression and TGF-β1, α-SMA mRNA levels more elevated, and Smad7 protein expression was significantly decreased compared with the four weeks, the difference was statistically significant (P lt; 0.05) ; Cai Pu Tang two weeks rats TGF-β1, α-SMA, Smad3, Smad4 protein expression and TGF-β1, α-SMA mRNA levels compared to the group of asthma two weeks significantly decreased, the difference was statistically There was significant (P lt; 0.05); But compared to the control group increased, the difference was not statistically significant (P gt; 0.05); Cai Pu Tang four weeks rats TGF-β1, α-SMA, Smad3, protein expression of Smad4 and TGF-β1, α-SMA mRNA levels compared to asthma group had significantly decreased four weeks, but compared to the control group increased, the difference was statistically significant (P lt; 0.05); Cai Pu Tang eight weeks rats TGF-β1, α-SMA, Smad3, Smad4 protein expression and TGF-β1, α-SMA mRNA levels compared to the group of asthmatic eight weeks significantly decreased, but compared to the control group increased, the difference was statistically significant (P lt; 0.05); while Smad7 protein expression compared to the asthma group significantly increased, but decreased compared to the control group, the difference was statistically significant (P lt ; 0.05). Conclusions: 1. Asthmatic airway remodeling in rats with prolonged excitation time, α-SMA expression gradually increased, the gradual thickening of airway smooth muscle. 2. TGF-β1/Smads pathway in airway remodeling in asthmatic rats models are dynamic process by which TGF-β1, Smad3, Smad4 and promote inlet remodeling, and Smad7 inhibition of airway remodeling. 3 Cai Pu Tang can inhibit airway remodeling in asthmatic rats, mechanisms may TGF-β1/Smads pathway.
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