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Objective To observe Naotaitong particle treatment of ischemic stroke effect, and initial treatment of ischemic stroke explore its mechanism . Methods ① choose similar weight SD rats 120 , male and female, divided into sham group , the placebo group, the experimental group and the positive control group . ② four groups of rats were fed high-fat diet after 10d , in addition to the sham group, the other three groups according to the literature produced by suture method cerebral artery model of focal cerebral ischemia (MCAO). ③ After the modeling , give the appropriate drugs and saline , continuous 28d. ④ respectively, after administration 7d, 10d, 14d, 21d, 28d five time points observed behavioral changes in rats , HE staining of rat brain neurons and immunohistochemical changes were detected in brain tissue of nerve growth factor Slit2 guide and vascular endothelial growth factor (VEGF) expression . Results ① various time points after treatment , the experimental rats neurological symptomatology score lower than the positive control group and were statistically significant between the two (P lt; 0.05). ② After modeling animals at each time point after ischemia are visible Slit2 staining positive cells peaked at 7d , higher than 10d, 14d, 21d, 28d. Slit2 protein experimental group were the highest, than in the placebo group and the positive control group , and the difference was statistically significant (P lt; 0.05). ③ In 7d, 10d, 14d time points , VEGF protein content in the experimental group compared with the positive control group, the difference was statistically significant (P lt; 0.05), and in the 21d, 28d time point , although the content of VEGF protein in the experimental group compared to positive in the control group , but the difference was not statistically significant (P gt; 0.05). ④ biopsy showed : the experimental group and positive control group compared with the placebo group 7d necrotic area decreased , 21d are visible capillaries surrounding the ischemic area increased in the experimental group and positive control groups showed no significant difference. Conclusion Slit2 after focal cerebral ischemia and VEGF expression increased after drug treatment significantly increased both in the experimental group than in the positive control group increased more significantly . Tip Naotaitong particles can promote hypoxic ischemic brain axonal growth and branch formation , promote endothelial regeneration and angiogenesis , to promote rehabilitation of limb function in rats and improve symptoms of neurological deficits , reduced the role of small cerebral infarction .
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