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Plant growth regulators are widely used in the production of modern agriculture, forestry, fruit trees, vegetables and flowers, has brought tremendous benefits to the people's production and life. With the increased awareness of environmental protection, it is not only focus on crop yields and nutrition, and more concerned about the impact on the environment and food safety issues, plant growth regulators for analysis and detection of great practical significance. The main subject of using high performance liquid chromatography (HPLC) and ultra-high-performance liquid chromatography - mass spectrometry (UPLC-MS/MS) study and the separation and analysis of three types of plant growth regulators, and the actual sample The three types of apple juice concentrate plant growth regulators content analysis determination. This paper mainly for the following three areas of study: 1. Established tune Forchlorfenuron, chlorine insects benzamide and diflubenzuron HPLC-UV separation and analysis methods, chromatographic separation column and mobile phase composition, detection wavelength chromatographic conditions optimization, 8 min to achieve separation and analysis of the three substances, the detection limits were 0.02μg/mL, 0.05μg/mL, 0.13μg/mL, recoveries were 92.4% -103.9%. Of concentrated apple juice three plant growth regulators quantitative analysis, the three substances content 0.15-5.32μg/kg. Through the chromatographic separation column, the mobile phase composition, detection wavelength, etc. Optimization of the chromatographic conditions, the establishment of indole butyric acid, fluorophenoxy acetic acid, alpha-naphthylacetic acid, and 2,4-D, HPLC-UV method for separation analysis the retention time of the four substances were 7.3 min, 8.3 min, 9.2 min, 12.0 min, the detection limit is to 0.01μg/mL 0.05μg/mL 0.01μg/mL, 0.05μg/mL; recoveries of 93.6 % -115.3%. Actual sample analysis, the content of the four substances in the sample 0.21-5.52μg/kg. Optimized DPC, CCC, fresh green pigment and MET UPLC-MS/MS conditions through the chromatography column and mobile phase composition, detection wavelength elution mode ion mode chromatography and mass spectrometry conditions optimization, quick analysis of the four substances, and the four substances by Mass Spectrometry parsing within 1.5 min. The detection limit of the four substances were 0.6μg/kg, 0.7μg/kg, 0.8μg/kg, 0.8μg/kg; recovery of 79.2% -103.6%. Actual sample analysis, the content of the four substances in the sample 0.83-4.29μg/kg.
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