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Pregnant chrysanthemum the Compositae chrysanthemum Chrysanthemum morifolium Ramat. Dried capitulum. Modern studies have shown that pregnant Chrysanthemum has good medical and health benefits. Volatile oil (essential oil), a class of plants or herbal aromatic odor, volatile oily liquid at room temperature, the general term for the main components of terpenes, sesquiterpenes and their oxygenated derivatives. This test pregnant daisy volatile oil extraction, chemical composition and cigarettes plus Hong carry out a more systematic study, and test results are as follows: 1. Steam distillation extract Huai Chrysanthemum volatile oil, study the crushing degree (mesh) plus water soak time and distillation time on Huai Chrysanthemum volatile oil obtained rate, test results showed that pregnant chrysanthemum volatile oil was rate the impact of the size of the order for the A (crushed degrees) gt; D (distillation time) gt; B (soak time) gt; C (add water). The variance analysis results showed that the The of A (crushed degrees) and D (distillation Time) on Huai Chrysanthemum volatile oil rates have a very significant impact, B (soak time) and C (plus water) results is not significant. The Huai Chrysanthemum volatile oil yield optimum extraction conditions were optimized by orthogonal experiment: crushed degrees 60-80 mesh, distillation time 11h, plus 15 times the amount of water soaking time of 10h. The blue color rendering of the volatile oil obtained by this method, the aroma pungent chrysanthemum yield was 0.267%. 2. Huai Chrysanthemum flash extraction of volatile oil, volatile oil extraction rate and quality of petroleum ether, ethyl acetate, acetone and n-hexane pregnant daisy, gas chromatography analysis of the results, and oil rate, color, shape and aroma to determine the conditions, to determine the best extraction solvent for the petroleum ether. On this basis, further through single factor test, Box-Behnken experimental study and response surface analysis to determine the stirring voltage is the main factor affecting pregnant chrysanthemum volatile oil yield, followed by extraction time, and finally the solid-liquid ratio, while identifying flash -extraction of optimum conditions: solid-liquid ratio 1:12, extraction time 35s, stirring voltage 200v, this method of volatile oil color yellow aroma micro chrysanthemum, pregnant daisy volatile oil was 1.55% . 3. Oil Soxhlet extraction Huai Chrysanthemum volatile, studied the ether, petroleum ether and n-hexane on the volatile oil extraction rate and quality of Huai Chrysanthemum and oil rate, color, shape and aroma to gas chromatographic analysis of the results, to determine the conditions determine the best extraction solvent ether. In this basis, further through single factor test, A Box-Behnken test research and response surface analysis, to determine the amount of solvent and extraction time pregnant chrysanthemum volatile oil extraction rate highly significant, extraction temperature on the extraction rate is not significant with optimum conditions, while Soxhlet extraction: solvent 10 times the amount of extraction temperature of 65 ° C, the extraction time is 7h, volatile oil obtained by this method showed pale yellow color, aroma as sweet, volatile oil rate of 1.98%. 4. Enzymatic extraction of Huai Chrysanthemum volatile oil research a plus mycophenolate amount, enzymatic hydrolysis time, pH and enzymatic reaction temperature Huai Chrysanthemum volatile oil yield, test results show that the order of the size of the impact of volatile oil yield Huai Chrysanthemum for C gt; B gt; A gt; D, namely: the pH value gt; enzymatic hydrolysis time gt; the enzyme dosage gt; enzymatic reaction temperature. Results of analysis of variance showed that, B (enzymatic hydrolysis time) and C (the pH value) Huai Chrysanthemum volatile oil have rates have significantly affected, factors A (plus mycophenolate amount) Huai Chrysanthemum volatile oil was rate of reach significantly with the level, factors D (the temperature of the enzymatic reaction) had no significant effect on the results. Huai Chrysanthemum volatile oil yield optimum extraction conditions were optimized by orthogonal experiment: the crude enzyme pregnant daisy quality ratio of 1:5, enzymatic hydrolysis time 9h, the pH value of 5, the enzymatic reaction temperature is 40 ° C, this method color rendering light yellow volatile oil aroma micro chrysanthemum and volatile oil yield was 0.98%. Huai Chrysanthemum 5.4 ways to extract the essential oil of main components for the β-phellandrene of poly parachutes ene, alpha-pinene, farnesol, nerolidol, cineol, daisy ketene, borneol, sabinene, α-the P. orientalis ene. According to GC-MS analysis, the assessment of the quality of the volatile oil, to determine the best extraction method for Soxhlet extraction. 7. The a single material tobaccosmoking filament Hong, Nanyang, Henan cut tobacco (C3F) plus Hong concentration of 0.50 ‰ -1.50 ‰, flue gas plump, good aroma. Delicate flue gas smoke coordination, reduced miscellaneous gas, irritant alleviate flavored concentration is further increased to 3.00 ‰, with incense smoke uncoordinated, masks incense smoke, Huai Chrysanthemum volatile oil smell; the Jilin Yanbian smoking tobacco (C3F) plus Hong concentration of 1.50 ‰ -3.00 ‰, aroma adequate, good aroma. comfortable aftertaste. 8. Cigarette Blend pipe tobacco Huai Chrysanthemum volatile oil, aroma volume increased significantly, good aroma qualitative change, improve the aroma quantity decline in miscellaneous gas and irritating, and taste more alcohol, comfortable, more appropriate amount of 0.50 ‰ -1.50 ‰.
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