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Lily is a perennial flower bulbs , is one of the important cut flowers in the world , with high ornamental value and economic value . From the lily breeding situation , the target of most of the work is to improve the ornamental characteristics , and the resistance breeding work is rarely reported . This study from the salt tolerance evaluation in vitro from Lily Lily salt tolerance study and the EMS mutation breeding two preliminary exploration results are as follows : 1 . Faster on the lily six cultivars and two wild species of tissue culture complex, a large number of tissue culture . Screening to determine the salt tolerance threshold , according to Lily 's growth performance under salt stress , these tissue culture medium by adding different concentrations of NaCl , result : ' bright yellow ' as 14g / L , ' Bernini ' 13g / L, ' truth ' and ' Marshal ' of 10g / L , 12g / L, ' Simplon ' , Yunnan wild lily , musk, lily ' Siberia ' . Tissue culture of different lily varieties or species in the same salt concentration coercion, random sampling , determination of physiological and biochemical indexes the content of MDA , SOD , POD , CAT activity changes to salt tolerance evaluation lily . According to the comprehensive evaluation of salt tolerance Sort : ' the dazzling yellow ' gt ; ' Bernini ' gt ; ' truth ' gt ; ' Marshal ' gt ; ' Simplon ' gt ; the Yunnan Wild Lily gt ; longiflorum gt; ' Siberia ' . 3 longiflorum callus was inoculated to a medium containing different concentrations of NaCl , periodic statistical survival rate the determine the callus Salt threshold value of 10 g / L, . 4 EMS solution was set up with different concentrations of the callus was the processing of the different time , found that the processing time is the same , the low concentration of EMS solution can improve the survival rate of callus , high concentration inhibits the growth of callus . EMS concentration as the treatment time , the decline in the survival rate of the callus . At the same time , to determine the 0.6% 2h for EMS longiflorum callus mutagenesis dose . 5 plants by the EMS mutagenesis callus differentiated into plant salt tolerance , salt tolerance enhanced variability . Determination of physiological and biochemical indices of these mutants , the results showed that the content of MDA has been lower than that of the control , the activity of SOD , POD activity has been higher than that of the control , CAT activity in the salt stress 9d is also higher than that of the control .
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