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Production due to lack of special and desirable Southern cultivation of fine strains of the southern climate, Breed weak, low yields, and the case of the hot and humid climate and easy flow ear, rotten bags and other issues, to solve these kinds of problems, the author initial screening through the domestication of wild black fungus Au053, Au083 strains, the study as a control on the southern main cultivated strains screened adapt to the southern regions, cultivation of fine strains Au053 and Au053 physiological characteristics such as temperature, moisture, pH and nutrient the study provides a theoretical basis for the Au053 cultivation techniques. The Au053 main cultivated strains studied differences in nutrition physiological characteristics from the nutritional physiological characteristics of flow ears rotten bag mechanism. The test results are as follows: 1, body cells pro and test, the results show that the Au053, Au083, the new Division No. 1, the new Section No. 5, Aul39 for different strains (new Section 1 Hao, pro and phenomena in the 5th, but by the enzymatic activity measurement identified as different strains). 2,5 hypha grew vitality Comparison of experimental results show that the Au053 the hyphae germination, colonization of the fastest growing stout thick, white, but the travel speed of the slowest. 3, the biological efficiency comparison test results show that, under the same cultivation conditions, the highest of Au053 production than the new Division 1 new Division No. 5, Au083, Au139, respectively, yield 10.08%, 8.89%, 20.15%, 22.86%, its differences. was highly significant. 4, flow ears, the rotten bags observed test results show that the Au053, Au083 not a flow ears, rotten bag phenomenon, while other strains flow ears, the rotten bags more serious. The ability of anti miscellaneous contrast test showed that the strongest, Au053 anti Miscellaneous capacity, the Au083 followed, other strains weaker. 6, Au053 physiological characteristics of study: Au053 mycelium germination temperature of 18 ℃, the growth temperature of 14-40 ℃, the optimum temperature of 25-29 ° C; mycelium in culture could grow material moisture content of 52% -68% range, the most appropriate moisture content of 59% -64%; mycelial growth optimum pH range of 5.0-7.5, the optimum pH of 6.0-6.5. 7, outside the main extracellular Au053 mycelial growth phase activity studies show: amylase, CMC the higher the initial activity of the enzyme, xylanase, laccase; amylase activity with increasing strain age upward trend after the 20d reached a maximum downward trend thereafter, the other three kinds of enzyme activity tended to decrease; paint activity changes very unique, almost no activity in strain age 20-40d, but the rapid recovery and a sharp increase in activity in strain age 40d, 60d activity reached nearly 2 times the initial stage of growth. Extracellular activity changes of mycelium growth stage starch first be utilized, followed by the cellulose and hemicellulose, lignin using less, with the starch and other non-wood cellulose and cellulose, hemicellulose utilization reduced With the recovery of laccase activity and a sharp rise late in the mycelial growth, lignin begins to decompose use, so as to ensure the continuous supply of nutrition, it mycelium to maintain its vitality and resistance to adverse environmental. 8, by cell outside activity and its trend on the the Au053 and Cultivars strain mycelium growth stage, the analysis of the physiological characteristics of their nutritional differences, from the angle of nutrition physiological characteristics of the mycelium germination, the colonization mechanism and flow ear, rotten bags mechanism.
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