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Bone marrow mesenchymal stem cells to differentiate into odontoblasts in vivo

Author: DuanQingYue
Tutor: LuQun；JinYan；LinZuo
School: Fourth Military Medical University
Course: Clinical Stomatology
Keywords: Green fluorescent protein transgenic mice Bone marrow mesenchymal stem cells Dentin defects Stem cell niches Reparative dentin DSP DMP-1
CLC: R78
Type: Master's thesis
Year: 2010
Downloads: 53
Quote: 0
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Abstract


Stem cells differentiate into odontoblasts dentin defects is the key. Mesenchymal stem cells are a type of adult stem cells, stem cell niches under the regulation can be directed to differentiate into a variety of mesodermal and neuroectodermal origin of the tissue cells, but for this non-tooth-derived stem cells can be passed to differentiate into odontoblasts Way To Fix dentin defects research rarely reported. In this study, the use of green fluorescent protein transgenic mice bone marrow mesenchymal stem cells in vivo observation BMMSCs can differentiate into odontoblasts and participate in dentin damage repair for tooth tissue engineering to provide more viable seed cells. Methods: (1) take the green fluorescent protein transgenic mice whole bone marrow, adherent cultured green fluorescent protein transgenic mice bone marrow mesenchymal stem cells, flow cytometry identified cell surface molecular markers, in vitro osteogenic adipogenic ; (2) Construction of green fluorescent tail vein injection of bone marrow mesenchymal stem cells in chimeric normal C57BL / 6 mouse model; (3) Preparation of chimeric mouse model of dentin defects, observed by immunofluorescence assay green fluorescent bone marrow Mesenchymal stem cells are involved in the repair of dentin defects explore bone marrow mesenchymal stem cells as seed cells repair the feasibility of dentin defects. Results: (1) develop a green fluorescent protein transgenic mice bone marrow mesenchymal stem cells are highly proliferative capacity and sustainable passaged, P3 cells by flow cytometry positive stem cell surface molecules, green fluorescent expression stability, in vitro differentiation adipogenic osteogenic capacity; (2) construct a chimeric mouse model of whole bone marrow and then cultured with a green fluorescent marker found a large number of cells exist, organizations have different organs number of green fluorescent cells present. (3) Immunohistochemistry Fluorescent Display chimeric mice dentin pulp cavity defect model a large number of spontaneous green fluorescent cells, part of dentin defects cells expressing green fluorescent protein dentin-specific DSP and DMP-1. Conclusion: The use of tracer ability of the green fluorescent protein transgenic mice bone marrow mesenchymal stem cells to build fluorescent chimeric mouse model, validation of bone marrow mesenchymal stem cells in vivo odontoblast differentiation to the ability of bone marrow mesenchymal found Mesenchymal stem cells in vivo microenvironment can be induced odontoblast differentiation and dentin defects involved in the repair of tooth tissue engineering can be used as source of stem cells.

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CLC: > Medicine, health > Oral Sciences
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