Dissertation > Excellent graduate degree dissertation topics show

Fermentation and Immunoreactivity of Extracellular Polysaccharide by P.baumii Pilát

Author: ShaoJie
Tutor: ZengXiaoXiong
School: Nanjing Agricultural College
Course: Of Food Science
Keywords: Medicinal fungi Phellinus baumii Pilát submerged culture immuno-stimulating activity central composite design response surface methodology
CLC: R285
Type: Master's thesis
Year: 2010
Downloads: 13
Quote: 0
Read: Download Dissertation


Medicinal fungus has been used as a traditional Chinese medicine in China for several thousand years because of their various biological and pharmacological active ingredients, especially, polysaccharides. Recently, polysaccharides from medicinal fungus have been well-known as a potential Biological response modifier. Modern pharmacological studies showed that medicinal fungi polysaccharides have immunostimulating and anti-tumor properties."Sanghuang", which has been used as a traditional Chinese medicine in China for a long time, has gained wide popularity as potential immunostimulating and anti-tumor properties and has become one of the valuable medicinal fungi in the world. Therefore, it deserves investigation to produce EPS from P. baumii Pilat by submerged culture and immunostimulating activities of EPS. The main results are shown as follows:1. A 2(7-3) fractional factorial design (FFD) was employed to determine the key ingredients significantly affecting EPS production from P. baumii Pilat. Seven nutrient factors considered for the design were glucose, peptone, yeast extract, diammonium oxalate monohydrate, KH2PO4, MgSO4 and thiamine (VB1). Among the variables screened, the three variables including glucose, yeast extract and diammonium oxalate monohydrate were determined as the most significant variables influencing EPS production.2. The levels of the significant parameters and interaction effects between various medium components which influence significantly the EPS production from FFD were analyzed and optimized by using a central composite design (CCD) in response surface methodology (RSM) and the response surface regression procedure. The optimal medium composition was determined as following (g/1):glucose 34.12, yeast extract 5.01, peptone 4, MgSO4 0.75, KH2PO4 1, VB1 0.0075 and diammonium oxalate monohydrate 0.88.3. The effect of EPS from P. baumii Pilat on the splenocyte proliferative response in vitro was investigated by using MTT-based colorimetric method. In the absence of mitogen, EPS at 100μg/ml enhanced the proliferation with the highest splenocyte proliferation index (1.18±0.09). Meantime, the results also showed that EPS could enhance both the LPS-induced B cells and Con A-induced T cells proliferation.4. The ability of EPS from P. baumii Pilat to stimulate acid phosphatase activity in peritoneal macrophages in vitro was investigated by using the cell model experiments. Results showed that the activities of acid phosphatase in macrophages increased significantly by EPS in a dose-dependent manner (25-200μg/ml). Furthermore. EPS at 200μg/ml activated the acid phosphatase activity by 105.50% of the control. These results suggested that the EPS from P. baumii Pilat strongly induced the activation of macrophage, increased significantly acid phosphatase activity in peritoneal macrophages. and possessed higher immunomodulatory potential.

Related Dissertations

  1. Studies on Extraction, Isolation, Purification and Antioxidation Activity of Flavonoids from the Fruits of Avicennia Marina,S793.9
  2. Study on Application of Non-phosphate Additive in Frozen Penaeus Vannamei and Mechanism of Water-holding,TS254.4
  3. The Combined Effect of Several Environmental Factor on Fertilization, Hatching and Juvenile Growth of Nile Tilapia,S917.4
  4. Modification for PVA-Based Composite Packaging Material with Nano-SiO2 and Its Influence on Fresh Preservation Effect of Salted Duck Eggs,TS253.46
  5. Optimization of Extraction of Gastrodin from Rhizoma Gastrodia by Response Surface Methodology,R284.1
  6. Screening of Pigment-free Pullulan High Producing Microorganism and Research of Optimal Culture Media,TQ920
  7. Study on Preparation Isolated Proteins and Active Peptides from Pumpkin Seeds,TS201.21
  8. Rubber - cobalt - nickel- Preparation of adhesion promoters,TQ430.1
  9. Biosynthesis of Polyphenols of Inonotus Obliquus in Submerged Cultures and Biological Activity,TQ920
  10. Based on the theory of constraints simulation and optimization of production operations,F273
  11. Hot stamping door impact beams Experimental studies and material properties gradient optimization,U467.3
  12. Semi- combustible cartridge gun seal structure design of multi-objective optimization,TJ303
  13. Planning for weapon delivery Adaptive Hybrid Response Surface Optimization Method,V271.4
  14. The Camptotheca acuminata endophytic fungi and their secondary metabolites,R284
  15. Solid-liquid system under the white rot fungi biodegradation studies hexachlorobenzene,X172
  16. The Study of Naphthalene Degradatrion Performance Using TiO2/Fe3O4-SiO2 Photocatalyst in Wastewater,X703
  17. Studies on Prefermented Frozen Dietary Fiber Dough and Its Application in Steamed Bread Making,TS213.2
  18. Preparation of microwave heating of direct reduced iron process,TF55
  19. Study on the Biodiesel from Rice Bran Oil in Supercritical Methanol,TE667
  20. Study on the Extraction of Natural β-Carotene and the Development of Its Soft Capsule Product,R151

CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology
© 2012 www.DissertationTopic.Net  Mobile