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Isolation of Phenol-Degrading Fungiand Preliminary Studies on Its Degrading Characteristics

Author: ZhouZuoZuo
Tutor: CaiWeiMin
School: Shanghai Jiaotong University
Course: Environmental Engineering
Keywords: Biodegradable Candida tropicalis Phenol Degradation characteristics Dynamics
CLC: X172
Type: Master's thesis
Year: 2011
Downloads: 108
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Abstract


Phenolic compounds is a the prototype quality highly toxic substances, has a three-induced effect of all life, the individual has a significant toxic effects. As a common industrial pollutant, phenol widespread in the paper, oil refining, coking, pesticides, chemical, pharmaceutical synthesis industry, industrial wastewater, untreated phenolic wastewater caused a serious threat to human survival environment. Biological method is a low-cost, smaller secondary pollution, industrial wastewater treatment methods, Chiang Kai-shek to the pure culture microbial treatment technology transformation from traditional mixed microbial processing technology to Phenolic industrial wastewater treatment stage. Research group in the preliminary studies found that good degradation characteristics of aerobic granular sludge for the simulation of phenol wastewater to study phenol degradation mechanism and thus strengthening its phenol degradation performance, this paper, separation and purification technology from aerobic granular sludge screening isolated two-plant height vitality phenol degrading bacteria, and by the the 23Sr DNA sequencing, strain Candida tropicalis (Candida tropicalis) case. The results show that: the strains A1 and A3 have relatively high phenol tolerance in phenol as the sole carbon source under culture conditions; also were able to tolerate 1 750 mg / L and 2 000 mg / L of phenol efficient phenol degradation rate in the inoculum of 3% and a temperature of 30 ° C, pH value of 6.85, with volume of 100 mL / 250 mL, kept shaking speed 120 r / min phenol-degrading process conditions, A1 can make the initial concentration of 1 000 mg / L of phenol 24 h degradation rate reached more than 95%, while the A3 can reach 100% of the degradation rate. Glucose on the microbial degradation of phenol the synergies and substrate competition between dosage ≤ 1.0 g / L; ammonium nitrate plus nitrogen source, cost-effective; simulated wastewater prepared already contain considerable amounts of inorganic trace elements but the removal of trace elements in liquid medium for phenol degradation also larger impact of trace elements are essential for microorganisms. Meanwhile, in the case of fully dissolved oxygen, Candida tropicalis Intrinsic kinetics of degradation of phenol, the strains Haldane cell growth kinetics equation parameters obtained by fitting the experimental data: Candida tropicalis A1 strains - the maximum specific growth rate μX max = 0 409h -1 , phenol half-saturation constant K S = 41.37mg / L, phenol inhibition constant K i = 387.41mg / L; corresponding phenol degradation kinetic parameters: the growth of the correlation coefficient A = 0.4387, non-growth correlation coefficient = 0.0003h -1 ; Candida tropicalis Candida the A3 strain - the largest growth rate μX max = 0 418h -1 , phenol half-saturation constant K S = 74.69mg / L phenol inhibition constant K i = 432.38mg / L; the corresponding phenol degradation kinetics parameters: the growth of the correlation coefficient A = 0.4872, non-growth correlation coefficient B = 0.0031h -1 . The strain is to arrive at a good value in engineering.

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