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Outbreaks of the Oriental Migratory Locust Locusta migratoria manilensis (Meyen) is our most widely distributed area, the most serious harm agricultural pests. Outbreaks of the Oriental Migratory Locust is also insect morphology, physiology, developmental biology, neurobiology important model organism for research in the field. Currently, the study locust life characterized at the molecular level has become a hot spot, there are a large number of genes have been cloned, and further research, the need for an effective method to interpret the function of these genes. With the development of the methods and techniques of gene function, RNA interference (RNAi) has become one of the main methods to study insect gene function. Orthodenticle (otd) gene during embryonic development in the the research fruit fly (Drosophila melanogaster). The Drosophila otd Bicoid (Bcd) activation former head zygotic gene expression and regulation of the head, as the section and tentacles Festival formation; otd red flour beetle (Tribolium castaneum) but maternal genes, maternal otd mRNA uniform distributed in ovo, only in the former head of the embryo to the blastocyst stage, the expression, regulation of the formation of the head body section. Otd gene is reported that there are bees (Apis mellifera) insects Calliphoridae pupae set gold wasp (Nasonia vitripennis), hover flies (Episyrphus balteatus), the Mediterranean real rope (Ceratitis capitata), pea aphid (Acyrthosiphon pisum ) and the Caribbean fruit fly (Anastrepha suspensa). Cloned two fragments of the Oriental migratory locust otd gene and embryo RNAi and parental RNAi approach preliminary study of the function of the gene. And to analyze and compare the advantages and disadvantages of the two methods is used to Oriental migratory locust gene function studies. Now the main research content and results are summarized as follows: ① the otd gene fragments of cloned by PCR, RT-PCR method to clone a the two Oriental migratory locust otd gene fragment, respectively: 91 bp and 235 bp. Among them, 91 bp fragment belongs to the sequence of the coding region, the sequence of the 235 bp fragment containing the non-coding region. Derivation and comparison of the amino acid sequence of the 91 bp sequence and found that it belongs to a the otd gene family; nucleotide sequence alignment of the 235 bp sequence found it high otd2 gene homology. ② Outbreaks of the Oriental Migratory Locust RNAi prepared by in vitro transcription dsRNA, dsRNA were injected to the of locust egg and female adult haemocoel. Injected dsRNA eggs, the survival rate is lower, with a significant difference compared with the control, injecting eggs easy to cause human injury; dsRNA-injected female adults survived began spawning after a week, compared with the control, The injected dsRNA female adult fecundity did not differ significantly dsRNA does not affect the fertility of female adults, and will not cause serious human injury. embryo RNAi can be used for the study of gene function in the Oriental migratory locust during embryonic development parentalRNAi There was no difference in the type of silence gene expression. Both in a dose-dependent, the dsRNA have no significant impact on the length of the embryo RNAi and parental RNA effect. The the Locusta migratoria otd gene parental RNAi works in days 6,7,8 after eclosion female adults, especially in the first seven days after emergence of RNAi effect is more significant. Embryo RNAi and parental RNAi otd gene silencing specific phenotype: embryos formed only three pairs of thoracic legs and abdomen of 10 abdominal segment, the former head of the single compound eyes, antennae section and section of intercalatum, jaw palate, upper lip, chin, lower lip and chest first, the second pair of pereiopods missing. ③ otd gene function generated according the Locusta migratoria otd Gene embryo RNAi and parental RNAi phenotype, locust otd gene region in the head and chest (T1 and T2) plays typical gap gene functions, regulation of the head, chest (T1 and T2) somite formation. otd genes may play a more important role in the formation of the the low insect body mode, the regulation of the number of somites more than fruit flies and other insects.
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